Zhaoqing Yan , Amit Eshed , Anli A. Tang , Nery R. Arevalos , Zachary M. Ticktin , Soma Chaudhary , Duo Ma , Griffin McCutcheon , Yudan Li , Kaiyue Wu , Sanchari Saha , Jonathan Alcantar-Fernandez , Jose L. Moreno-Camacho , Abraham Campos-Romero , James J. Collins , Peng Yin , Alexander A. Green
{"title":"Rapid, multiplexed, and enzyme-free nucleic acid detection using programmable aptamer-based RNA switches","authors":"Zhaoqing Yan , Amit Eshed , Anli A. Tang , Nery R. Arevalos , Zachary M. Ticktin , Soma Chaudhary , Duo Ma , Griffin McCutcheon , Yudan Li , Kaiyue Wu , Sanchari Saha , Jonathan Alcantar-Fernandez , Jose L. Moreno-Camacho , Abraham Campos-Romero , James J. Collins , Peng Yin , Alexander A. Green","doi":"10.1016/j.chempr.2024.03.015","DOIUrl":null,"url":null,"abstract":"<div><p>Rapid, simple, and low-cost diagnostic technologies are crucial tools for combating infectious disease. We describe a class of aptamer-based RNA switches, or aptaswitches, that recognize target nucleic acid molecules and initiate the folding of a reporter aptamer. Aptaswitches can detect virtually any sequence and provide an intense fluorescent readout without intervening enzymes, generating signals in as little as 5 min and enabling detection by eye with minimal equipment. Aptaswitches can be used to regulate the folding of seven fluorogenic aptamers, providing a general means of controlling aptamers and an array of multiplexable reporter colors. By coupling isothermal amplification reactions with aptaswitches, we reach sensitivities down to 1 RNA copy/μL in one-pot reactions. Application of multiplexed all-in-one reactions against RNA from clinical saliva samples yields an overall accuracy of 96.67% for detection of SARS-CoV-2 in 30 min. Aptaswitches are thus versatile tools for nucleic acid detection that are readily integrated into rapid diagnostic assays.</p></div>","PeriodicalId":268,"journal":{"name":"Chem","volume":null,"pages":null},"PeriodicalIF":19.1000,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chem","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2451929424001244","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Rapid, simple, and low-cost diagnostic technologies are crucial tools for combating infectious disease. We describe a class of aptamer-based RNA switches, or aptaswitches, that recognize target nucleic acid molecules and initiate the folding of a reporter aptamer. Aptaswitches can detect virtually any sequence and provide an intense fluorescent readout without intervening enzymes, generating signals in as little as 5 min and enabling detection by eye with minimal equipment. Aptaswitches can be used to regulate the folding of seven fluorogenic aptamers, providing a general means of controlling aptamers and an array of multiplexable reporter colors. By coupling isothermal amplification reactions with aptaswitches, we reach sensitivities down to 1 RNA copy/μL in one-pot reactions. Application of multiplexed all-in-one reactions against RNA from clinical saliva samples yields an overall accuracy of 96.67% for detection of SARS-CoV-2 in 30 min. Aptaswitches are thus versatile tools for nucleic acid detection that are readily integrated into rapid diagnostic assays.
期刊介绍:
Chem, affiliated with Cell as its sister journal, serves as a platform for groundbreaking research and illustrates how fundamental inquiries in chemistry and its related fields can contribute to addressing future global challenges. It was established in 2016, and is currently edited by Robert Eagling.