Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla)

IF 2.5 3区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Fish Physiology and Biochemistry Pub Date : 2024-04-19 DOI:10.1007/s10695-024-01338-1

Marta Blanes-García, Zoran Marinović, Juan Germán Herranz-Jusdado, Xuan Xie, Leonor Ferrão, Victor Gallego, Luz Pérez, Abdul Rasheed Baloch, Ákos Horváth, Martin Pšenička, Juan F. Asturiano, Marina Morini

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Abstract

Identification of specific molecular markers for spermatogonial stem cells in teleost is crucial for enhancing the efficacy of reproductive biotechnologies in aquaculture, such as transplantation and surrogate production in fishes. Since it is not yet possible to distinguish spermatogonial stem cells of European eel (Anguilla anguilla) using specific molecular markers, we isolated spermatogonial cells from immature European eels to find these potential markers. We attempted this by studying three candidate genes: vasa, nanos2, and dnd1. Two vasa (vasa1 and vasa2) genes, nanos2, and dnd1 were identified, characterized, and studied in the muscle, testis, and isolated spermatogonia. Our results showed that vasa1 and vasa2 had the highest levels of expression when measured by qPCR. In situ hybridization and immunochemistry assays showed that the four genes were localized explicitly in type A spermatogonia. However, vasa1 and vasa2 exhibited stronger signals in the immature testicular tissue than the other two potential markers. According to this, vasa1 and vasa2 were found to be the most effective markers for spermatogonial cells in the European eel.

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欧洲鳗鲡精原细胞潜在生物标志基因的特征描述

鉴定远洋鱼类精原干细胞的特异性分子标记对于提高水产养殖中生殖生物技术（如鱼类移植和代孕生产）的效率至关重要。由于目前还无法使用特定的分子标记来区分欧洲鳗鲡的精原干细胞，我们从未成熟的欧洲鳗鲡体内分离出精原细胞，以寻找这些潜在的标记。为此，我们研究了三个候选基因：vasa、nanos2 和 dnd1。我们在肌肉、睾丸和分离的精原细胞中鉴定、描述和研究了两个 vasa（vasa1 和 vasa2）基因、nanos2 和 dnd1。结果表明，通过 qPCR 测量，vasa1 和 vasa2 的表达水平最高。原位杂交和免疫化学检测表明，这四个基因明确定位于 A 型精原细胞。然而，与其他两个潜在标记相比，vasa1 和 vasa2 在未成熟睾丸组织中表现出更强的信号。因此，vasa1 和 vasa2 被认为是欧洲鳗精原细胞的最有效标记。

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来源期刊

Fish Physiology and Biochemistry 农林科学-生化与分子生物学

CiteScore

5.60

自引率

6.90%

发文量

106

审稿时长

4 months

期刊介绍： Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.