In vivo SPECT imaging of Tc-99m radiolabeled exosomes from human umbilical-cord derived mesenchymal stem cells in small animals

IF 4.1 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomedical Journal Pub Date : 2024-04-16 DOI:10.1016/j.bj.2024.100721

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Abstract

Extracellular vesicles derived from human umbilical cord-derived mesenchymal stem cells (UCMSC-EVs) have been postulated to have therapeutic potential for various diseases. However, the biodistribution and pharmacokinetics of these vesicles are still unclear. For a better understanding of the in vivo properties of UCMSC-EVs, in the present study, these vesicles were first radiolabeled with Technetium-99m (^99mTc-UCMSC-EVs) and evaluated using in vivo single photon emission computed tomography (SPECT) imaging and biodistribution experiments. SPECT images demonstrated that the liver and spleen tissues mainly took up the ^99mTc-UCMSC-EVs. The biodistribution study observed slight uptake in the thyroid and stomach, indicating that ^99mTc-UCMSC-EVs was stable at 24 h in vivo. The pharmacokinetic analyses of the blood half-life demonstrated the quick distribution phase (0.85 ± 0.28 min) and elimination phase (25.22 ± 20.76 min) in mice. This study provides a convenient and efficient method for ^99mTc-UCMSC-EVs preparation without disturbing their properties. In conclusion, the biodistribution, quick elimination, and suitable stability in vivo of ^99mTc-UCMSC-EVs were quantified by the noninvasive imaging and pharmacokinetic analyses, which provides useful information for indication selection, dosage protocol design, and toxicity assessment in future applications.

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人脐带间充质干细胞Tc-99 m放射性标记外泌体在小动物体内的SPECT成像。

从人脐带间充质干细胞（UCMSC-EVs）中提取的细胞外囊泡被认为具有治疗各种疾病的潜力。然而，这些囊泡的生物分布和药代动力学尚不清楚。为了更好地了解 UCMSC-EVs 的体内特性，本研究首先用锝-99m（99mTc-UCMSC-EVs）对这些囊泡进行了放射性标记，并使用体内单光子发射计算机断层扫描（SPECT）成像和生物分布实验对其进行了评估。SPECT 图像显示，肝脏和脾脏组织主要吸收了 99m锝-UCMSC-EVs。生物分布研究观察到甲状腺和胃有轻微摄取，表明 99mTc-UCMSC-EVs 在体内 24 小时内是稳定的。血液半衰期的药代动力学分析表明，99mTc-UCMSC-EVs 在小鼠体内的分布期（0.85 ± 0.28 分钟）和消除期（25.22 ± 20.76 分钟）均较快。该研究为制备 99mTc-UCMSC-EVs 提供了一种方便、高效且不会干扰其特性的方法。总之，该研究通过无创成像和药代动力学分析，量化了 99mTc-UCMSC-EVs 在体内的生物分布、快速消除和适宜的稳定性，为未来应用中的适应症选择、剂量方案设计和毒性评估提供了有用信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Journal Medicine-General Medicine

CiteScore

11.60

自引率

1.80%

发文量

128

审稿时长

42 days

期刊介绍： Biomedical Journal publishes 6 peer-reviewed issues per year in all fields of clinical and biomedical sciences for an internationally diverse authorship. Unlike most open access journals, which are free to readers but not authors, Biomedical Journal does not charge for subscription, submission, processing or publication of manuscripts, nor for color reproduction of photographs. Clinical studies, accounts of clinical trials, biomarker studies, and characterization of human pathogens are within the scope of the journal, as well as basic studies in model species such as Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Mus musculus revealing the function of molecules, cells, and tissues relevant for human health. However, articles on other species can be published if they contribute to our understanding of basic mechanisms of biology. A highly-cited international editorial board assures timely publication of manuscripts. Reviews on recent progress in biomedical sciences are commissioned by the editors.