Vaspin facilitates the proliferation and osteogenic differentiation of periodontal ligament stem cells

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of periodontal research Pub Date : 2024-05-03 DOI:10.1111/jre.13254

Zhihui Jing, Xinran Feng, Xin Li, Xiaoyu Zhang, Chunling Pan

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Abstract

Objective

To investigate whether visceral adipose tissue-derived serine protease inhibitor (vaspin) can alleviate the inhibitory effect of high-glucose (HG) culture on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (PDLSCs) and to preliminarily explore the underlying mechanisms.

Background

High glucose produces damage to the regeneration of periodontal tissue of PDLSCs. The expression level of vaspin in periodontal tissue is high in periodontitis patients and effectively reduced after initial therapy of periodontal diseases. However, the effect of vaspin on PDLSCs remains unknown.

Materials and Methods

PDLSCs were cultured in media augmented with 5.5 or 25.0 mM concentrations of glucose to elucidate the impact and mechanism of vaspin on PDLSCs under high glucose in vitro. Proliferation was measured by Cell Counting Kit-8 (CCK8) assay. Osteogenesis of PDLSCs was assessed by alkaline phosphatase (ALP) staining, ALP activity, and Alizarin Red staining. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blot (WB) were used to investigate the osteo-specific markers. Then, the molecular impact of vaspin in the presence/absence of HG on PDLSCs physiology was determined with TGF-β1/Smad signaling pathway as the main focus.

Results

It was revealed that the proliferation and osteogenic differentiation (OD) of PDLSCs under HG was reduced, and by adding vaspin the anti-osteogenic impact of HG was relieved. Moreover, vaspin enhanced TGF-β1/Smad signaling pathway activity. Pretreatment with TGF-β1 inhibitor blocked vaspin-triggered TGF-β1/Smad signal activation and minimized the vaspin-induced protective effect against HG-inhibited growth and OD.

Conclusions

In summary, vaspin observably reduces HG-mediated inhibition of PDLSCs OD by modulating the TGF-β1/Smad signaling pathway. Vaspin may be a potential therapeutic for periodontal tissue regeneration in diabetic patients.

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Vaspin 促进牙周韧带干细胞的增殖和成骨分化

目的研究内脏脂肪组织衍生的丝氨酸蛋白酶抑制剂（vaspin）能否减轻高葡萄糖（HG）培养对人牙周韧带干细胞（PDLSCs）增殖和成骨分化的抑制作用，并初步探讨其潜在的机制。牙周炎患者牙周组织中 vaspin 的表达水平较高，牙周病初步治疗后，vaspin 的表达水平会有效降低。材料与方法在添加了 5.5 或 25.0 mM 浓度葡萄糖的培养基中培养 PDLSCs，以阐明 vaspin 在体外高糖条件下对 PDLSCs 的影响和机制。增殖用细胞计数试剂盒-8（CCK8）测定。通过碱性磷酸酶（ALP）染色、ALP活性和茜素红染色评估PDLSCs的成骨情况。定量实时聚合酶链反应（qRT-PCR）和免疫印迹（WB）用于研究骨特异性标志物。结果表明，在HG作用下，PDLSCs的增殖和成骨分化（OD）降低，而加入vaspin后，HG的抗成骨影响得到缓解。此外，vaspin 还能增强 TGF-β1/Smad 信号通路的活性。结论综上所述，vaspin通过调节TGF-β1/Smad信号通路，明显降低了HG介导的对PDLSCs OD的抑制。Vaspin可能是糖尿病患者牙周组织再生的一种潜在疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.

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