HA2-FimA DNA Vaccine Treats Experimental Periodontitis.

IF 1.4 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Oral health & preventive dentistry Pub Date : 2024-04-30 DOI:10.3290/j.ohpd.b5281939

Huijie Zhang, Yueyue Wang, Zhu Wang, Nanqing Fu, Xinrui Wang, Guohui Bai

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Abstract

Purpose: To study the therapeutic effect of hemagglutinin-2 and fimbrial (HA2-FimA) vaccine on experimental periodontitis in rats.

Materials and methods: The first batch of rats was divided into two groups and immunised with pure water or pVAX1-HA2-FimA at the age of 6, 7, and 9 weeks. After sacrificing the animals, total RNA was extracted from the spleens for RNA high-throughput sequencing (RNA-Seq) analysis. The second batch of rats was divided into four groups (A, B, C, D), and an experimental periodontitis rat model was established by suturing silk thread around the maxillary second molars of rats in groups B, C, and D for 4 weeks. The rats were immunised with pure water, pVAX1-HA2-FimA vaccine, empty pVAX1 vector, and pure water at 10, 11, and 13 weeks of age, respectively. Secretory immunoglobulin A (SIgA) antibodies and cathelicidin antimicrobial peptide (CAMP) levels in saliva were measured by enzyme-linked immunosorbent assay (ELISA). All rats were euthanised at 17 weeks of age, and alveolar bone loss was examined using micro-computed tomography (Micro-CT).

Results: Through sequencing analysis, six key genes, including Camp, were identified. Compared with the other three groups, the rats in the periodontitis+pVAX1-HA2-FimA vaccine group showed higher levels of SIgA and CAMP (p < 0.05). Micro-CT results showed significantly less alveolar bone loss in the periodontitis+pVAX1-HA2-FimA vaccine group compared to the periodontitis+pVAX1 group and periodontitis+pure water group (p < 0.05).

Conclusion: HA2-FimA DNA vaccine can increase the levels of SIgA and CAMP in the saliva of experimental periodontitis model rats and reduce alveolar bone loss.

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HA2-FimA DNA 疫苗可治疗实验性牙周炎。

目的：研究血凝素-2和fimbrial（HA2-FimA）疫苗对大鼠实验性牙周炎的治疗效果：第一批大鼠分为两组，分别在 6、7 和 9 周龄时用纯水或 pVAX1-HA2-FimA 进行免疫接种。牺牲动物后，从脾脏中提取总 RNA 进行 RNA 高通量测序（RNA-Seq）分析。将第二批大鼠分为四组（A、B、C、D），在 B、C、D 组大鼠上颌第二磨牙周围缝合丝线 4 周，建立实验性牙周炎大鼠模型。在大鼠 10、11 和 13 周龄时分别用纯水、pVAX1-HA2-FimA 疫苗、空 pVAX1 载体和纯水对其进行免疫接种。通过酶联免疫吸附试验（ELISA）测定唾液中分泌型免疫球蛋白 A（SIgA）抗体和柔毛素抗菌肽（CAMP）水平。所有大鼠均在 17 周龄时安乐死，并使用显微计算机断层扫描（Micro-CT）检查牙槽骨损失：结果：通过测序分析，确定了包括 Camp 在内的六个关键基因。与其他三组相比，牙周炎+pVAX1-HA2-FimA 疫苗组大鼠的 SIgA 和 CAMP 水平更高（p < 0.05）。显微 CT 结果显示，与牙周炎+pVAX1 组和牙周炎+纯水组相比，牙周炎+pVAX1-HA2-FimA 疫苗组的牙槽骨损失明显较少（P < 0.05）：结论：HA2-FimA DNA 疫苗能提高实验性牙周炎模型大鼠唾液中 SIgA 和 CAMP 的水平，减少牙槽骨流失。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral health & preventive dentistry DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Clinicians, general practitioners, teachers, researchers, and public health administrators will find this journal an indispensable source of essential, timely information about scientific progress in the fields of oral health and the prevention of caries, periodontal diseases, oral mucosal diseases, and dental trauma. Central topics, including oral hygiene, oral epidemiology, oral health promotion, and public health issues, are covered in peer-reviewed articles such as clinical and basic science research reports; reviews; invited focus articles, commentaries, and guest editorials; and symposium, workshop, and conference proceedings.