Application of a laparoscopic device for cell-derived sheet transplantation on the liver in a porcine model.

IF 1.7 4区医学 Q2 SURGERY Minimally Invasive Therapy & Allied Technologies Pub Date : 2024-08-01 Epub Date: 2024-04-09 DOI:10.1080/13645706.2024.2328610

Keisuke Toya, Yoshito Tomimaru, Shogo Kobayashi, Kiyokazu Nakajima, Akima Harada, Kazuki Sasaki, Yoshifumi Iwagami, Daisaku Yamada, Takehiro Noda, Hidenori Takahashi, Koichi Hayakawa, Isamu Matsuda, Takahiro Naka, Shigeru Miyagawa, Yuichiro Doki, Hidetoshi Eguchi

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Abstract

Background: Cell-derived sheets are of global interest for regenerative therapy. Transplanting a sheet for abdominal organs requires a device for laparoscopic delivery to minimize invasiveness. Here, using a porcine model, we aimed to confirm the feasibility of a device developed to deliver sheets to the thoracic cavity in a laparoscopic transplantation procedure.

Material and methods: We used the device to transplant human skeletal myoblast cell sheets onto the liver and measured extra-corporeal, intra-abdominal, and total procedure times for sheet transplantation. Tissues, including the liver and the sheet, were collected two days after transplantation and analyzed histologically.

Results: In all experiments (n = 27), all sheets were successfully placed at target locations. The mean (± standard deviation) extra-corporeal, intra-abdominal, and total procedure times were 44 ± 29, 33 ± 12, and 77 ± 36 s, respectively. We found no difference between the two surgeons in procedure times. Histological analyses showed no liver damage with the transplantation and that sheets were transplanted closely onto the liver tissue without gaps.

Conclusion: We confirmed the feasibility of a simple universal device to transplant cell-derived sheets via laparoscopic surgery. This device could support a minimally invasive procedure for sheet transplantation.

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应用腹腔镜设备在猪模型肝脏上进行细胞衍生片移植。

背景：细胞衍生薄片是全球关注的再生疗法。为腹腔器官移植片状细胞需要一种腹腔镜输送装置，以最大限度地减少创伤。在此，我们使用猪模型，旨在证实在腹腔镜移植手术中将薄片输送到胸腔的装置的可行性：我们使用该装置将人类骨骼肌母细胞片移植到肝脏上，并测量了移植片的体外时间、腹腔内时间和总手术时间。移植两天后收集包括肝脏和细胞片在内的组织，并进行组织学分析：结果：在所有实验中（n = 27），所有肝片都成功放置在目标位置。体外、腹腔内和总手术时间的平均值（± 标准差）分别为 44 ± 29 秒、33 ± 12 秒和 77 ± 36 秒。我们发现两位外科医生的手术时间没有差异。组织学分析表明，移植手术没有造成肝脏损伤，移植片紧贴肝组织，没有缝隙：我们证实了通过腹腔镜手术移植细胞衍生薄片的简单通用设备的可行性。结论：我们证实了通过腹腔镜手术移植细胞衍生薄片的简单通用装置的可行性，该装置可支持微创薄片移植手术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Minimally Invasive Therapy & Allied Technologies 医学-外科

CiteScore

3.80

自引率

5.90%

发文量

审稿时长

6-12 weeks

期刊介绍： Minimally Invasive Therapy and Allied Technologies (MITAT) is an international forum for endoscopic surgeons, interventional radiologists and industrial instrument manufacturers. It is the official journal of the Society for Medical Innovation and Technology (SMIT) whose membership includes representatives from a broad spectrum of medical specialities, instrument manufacturing and research. The journal brings the latest developments and innovations in minimally invasive therapy to its readers. What makes Minimally Invasive Therapy and Allied Technologies unique is that we publish one or two special issues each year, which are devoted to a specific theme. Key topics covered by the journal include: interventional radiology, endoscopic surgery, imaging technology, manipulators and robotics for surgery and education and training for MIS.