Concurrent infection of dengue virus with malaria parasites among outpatients attending healthcare facilities in Benin city, Nigeria.

Porto biomedical journal Pub Date : 2024-04-15 eCollection Date: 2024-03-01 DOI:10.1097/j.pbj.0000000000000249

Joy Zitgwai Saidu, Rachel Obhade Okojie

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Abstract

Background: Dengue virus (DENV) and malaria parasites (MP) are among the common febrile diseases affecting the tropics and subtropics of the world. Both are mosquito-borne pathogens affecting humans and other animals.

Methods: Blood samples were collected from 280 consented out-patients attending the selected hospitals and were analyzed. Malaria parasites were detected using microscopy and Malaria Ag Pf/Pan Rapid Test Device. Dengue virus was detected by serology and heminested reverse transcriptase PCR (hnRT-PCR) to target the flavivirus polymerase (NS5) gene.

Results: Malaria parasites recorded a total positivity of 151 patients (53.9%) using microscopy, while DENV antibodies (DENV IgM and DENV IgG) were positive in 16 (5.7%) and 39 (13.9%) patients, respectively. There was a concurrent infection between MP/DENV IgM in 13 (4.6%) patients and MP/DENV IgG in 27 (9.6%) patients. Molecular identification revealed DENV serotype 2 in circulation.

Conclusion: This study documents molecular evidence of dengue virus coexisting with malaria parasites in the study population, hence the need for efficient surveillance and control system.

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尼日利亚贝宁市医疗机构门诊患者登革热病毒与疟原虫并发感染情况。

背景：登革热病毒（DENV）和疟原虫（MP）是影响世界热带和亚热带地区的常见发热疾病。这两种疾病都是由蚊子传播的病原体，影响人类和其他动物：方法：从选定医院的 280 名同意就诊的门诊病人身上采集血液样本并进行分析。使用显微镜和疟疾 Ag Pf/Pan 快速检测仪检测疟疾寄生虫。登革热病毒通过血清学和针对黄病毒聚合酶（NS5）基因的螯合反转录酶聚合酶链式反应（hnRT-PCR）进行检测：通过显微镜检查，151 名患者（53.9%）的疟原虫呈阳性，而 DENV 抗体（DENV IgM 和 DENV IgG）呈阳性的患者分别为 16 名（5.7%）和 39 名（13.9%）。13例（4.6%）患者的MP/DENV IgM和27例（9.6%）患者的MP/DENV IgG同时感染。分子鉴定显示，血液循环中的 DENV 血清型为 2：本研究提供了研究人群中登革病毒与疟原虫共存的分子证据，因此需要建立有效的监测和控制系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Porto biomedical journal

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