Bacteroides vicugnae sp. nov. isolated from the fecal material of an alpaca

IF 2.5 3区生物学 Q3 MICROBIOLOGY Anaerobe Pub Date : 2024-05-07 DOI:10.1016/j.anaerobe.2024.102862

Samuel Miller , Meredith Hendry , Jacobey King , Krithivasan Sankaranarayanan , Paul A. Lawson

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Abstract

Two strictly anaerobic, Gram-stain-negative rod-shaped bacterial isolates, A2-P53^T and A1-P5, were isolated from an enrichment of fecal material from two alpacas (Vicugna pacos). Based on a comparative 16S rRNA gene sequence analysis, the isolates were assigned to the genus Bacteroides with the highest sequence similarities to Bacteroides koreensis YS-aM39T (A2- P53T 97.7 % and A1-P5 97.9 %). Additionally, the average nucleotide identity and digital DNA-DNA hybridization values between these isolates and their closest relatives within Bacteroides were less than 92.1 % and 49.1 %, respectively. The average nucleotide identity between isolates A2-P53^T and A1-P5 was 99.9 %. The predominant cellular fatty acid for isolates A2-P53^T and A1-P5 was C_15:0 antesio. The G+C % content of the isolates was 41.7 %. Based on biochemical, phylogenetic, genotypic, and chemotaxonomic criteria, these isolates A2-P53^T and A1-P5 represent two individual strains of a novel species within the genus Bacteroides for which the name Bacteroides vicugnae sp. nov. is proposed. The type strain of this species is strain A2-P53^T (CCUG 77273^T = CCM 9377^T = NRRL B-65693^T).

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从羊驼排泄物中分离出的 Bacteroides vicugnae sp.

从两只羊驼（Vicugna pacos）的粪便富集物中分离出两株严格厌氧、革兰氏染色阴性的杆状细菌分离物 A2-P53T 和 A1-P5。根据 16S rRNA 基因序列比较分析，这些分离物被归入乳杆菌属，与 Bacteroides koreensis YS-aM39T 的序列相似度最高（A2- P53T 97.7% 和 A1-P5 97.9%）。此外，这些分离物与其在 Bacteroides 中的近亲之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别小于 92.1%和 49.1%。A2-P53T 和 A1-P5 分离物之间的平均核苷酸同一性为 99.9%。A2-P53T 和 A1-P5 分离物的主要细胞脂肪酸为 C15:0 antesio。分离物的 G+C % 含量为 41.7%。根据生化、系统发育、基因型和化学分类学标准，这些分离物 A2-P53T 和 A1-P5 代表了乳酸菌属中一个新物种的两株菌株，并将其命名为 Bacteroides vicugnae sp.nov.。该物种的模式菌株是菌株 A2-P53T（CCUG 77273T=CCM 9377T=NRRL B-65693T）。

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.