Modelo de cocultivo 3D in vitro de células endoteliales y vasculares de músculo liso humanas para el estudio del remodelado vascular patológico

IF 1.9 Q3 PERIPHERAL VASCULAR DISEASE Clinica e Investigacion en Arteriosclerosis Pub Date : 2024-11-01 DOI:10.1016/j.arteri.2024.03.007
Irene San Sebastián-Jaraba , María José Fernández-Gómez , Rafael Blázquez-Serra , Sandra Sanz-Andrea , Luis Miguel Blanco-Colio , Nerea Méndez-Barbero
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Abstract

Pathological vascular remodeling of the vessel wall refers to the structural and functional changes of the vessel wall that occur in response to injury that eventually leads to cardiovascular disease. The vessel wall is composed of two main types of cells, endothelial cells and vascular smooth muscle cells, whose communication is crucial in both the development of the vasculature and the homeostasis of mature vessels. Changes in the dialogue between endothelial cells and vascular smooth muscle cells are associated with various pathological states that triggers remodeling of the vascular wall. For many years, considerable efforts have been made to develop effective diagnoses and treatments for these pathologies by studying their mechanisms in both in vitro and in vivo models. Compared to animal models, in vitro models can provide great opportunities to obtain data in a more homogeneous, economical and massive way, providing an overview of the signaling pathways responsible for these pathologies. The implementation of three-dimensional in vitro co-culture models for the study of other pathologies has been postulated as a potentially applicable methodology, which determines the importance of its application in studies of cardiovascular diseases. In this article we present a method for culturing human endothelial cells and vascular smooth muscle cells, grown under non-adherent conditions, that generate three-dimensional spheroidal structures with greater physiological equivalence to in vivo conditions. This in vitro modeling could be used as a study tool to identify cellular and molecular mechanisms involved in the pathological processes underlying vascular remodeling.
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体外三维人内皮细胞和平滑肌细胞共培养模型,用于研究病理性血管重塑。
血管壁的病理性血管重塑是指血管壁的结构和功能因损伤而发生变化,最终导致心血管疾病。血管壁主要由两类细胞组成,即内皮细胞和血管平滑肌细胞,它们之间的交流对血管的发育和成熟血管的平衡至关重要。内皮细胞和血管平滑肌细胞之间对话的变化与引发血管壁重塑的各种病理状态有关。多年来,人们一直致力于通过在体外和体内模型中研究这些病理机制来开发有效的诊断和治疗方法。与动物模型相比,体外模型能以更均匀、更经济、更大规模的方式获取数据,为了解导致这些病症的信号通路提供了绝佳机会。三维体外共培养模型被认为是研究其他病理的一种潜在适用方法,这决定了它在心血管疾病研究中应用的重要性。在这篇文章中,我们介绍了一种在非粘附条件下培养人内皮细胞和血管平滑肌细胞的方法,这种方法能生成三维球形结构,与体内条件具有更高的生理等效性。这种体外建模可作为一种研究工具,用于确定血管重塑的病理过程所涉及的细胞和分子机制。
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来源期刊
Clinica e Investigacion en Arteriosclerosis
Clinica e Investigacion en Arteriosclerosis PERIPHERAL VASCULAR DISEASE-
CiteScore
3.20
自引率
6.20%
发文量
44
审稿时长
40 days
期刊介绍: La publicación idónea para acceder tanto a los últimos originales de investigación como a formación médica continuada sobre la arteriosclerosis y su etiología, epidemiología, fisiopatología, diagnóstico y tratamiento. Además, es la publicación oficial de la Sociedad Española de Arteriosclerosis.
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