Detection of clfA, clfB and coa genes in Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from Nasal Cavity of Cows, Buffalo and their Breeders in Nineveh Governorate, Iaq

H. Rahma, Dh. Jwher
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Abstract

The present study aimed to isolate and identify Methicillin-Resistant Staphylococcus aureus MRSA from the nasal cavity of healthy cows and buffaloes and their breeders in Nineveh Governorate and detect some virulence factors by using molecular methods. A total of 150 samples of cotton swabs were collected randomly from different areas of Nineveh governorate. The samples were cotton swabs from the nasal passages of healthy cattle, buffaloes, and their breeders (50 swabs of each type). All the samples were subjected to culture and molecular testing. The results showed the highest isolation percentage of S. aureus from cattle followed by breeders, then buffaloes, at 54%, 40%, and 32%, respectively. The total isolation percentage of MRSA was 65.1%. The highest percentage was in buffaloes, followed by breeders and cattle, at 93.75%, 70%, and 44.44%, respectively. Out of 41 isolates from cattle, buffaloes, and their breeders, the virulence genes clf A, clf B, and coa were detected in MRSA at rates of 100%, 80.49%, and 65.85%, respectively. The current study concluded that cattle and buffalo are considered carriers and potential transmitters of MRSA, which makes them risk factors for infection in humans, especially those who are in direct contact with animals. Together, these findings also highlight the need to prevent the transmission of zoonotic pathogens to humans via occupational exposure or consumption of contaminated animal products.
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检测从伊拉克尼尼微省奶牛、水牛及其饲养者鼻腔中分离出的耐甲氧西林金黄色葡萄球菌(MRSA)的 clfA、clfB 和 coa 基因
本研究旨在从尼尼微省健康奶牛和水牛及其饲养者的鼻腔中分离和鉴定耐甲氧西林金黄色葡萄球菌 MRSA,并采用分子方法检测一些毒力因子。研究人员从尼尼微省的不同地区随机采集了 150 份棉拭子样本。这些棉拭子样本来自健康牛、水牛及其饲养者的鼻腔(每种类型各 50 个棉拭子样本)。所有样本都进行了培养和分子检测。结果显示,牛的金黄色葡萄球菌分离率最高,其次是种牛,然后是水牛,分别为 54%、40% 和 32%。MRSA 的总分离率为 65.1%。水牛的比例最高,其次是种牛和牛,分别为 93.75%、70% 和 44.44%。在来自牛、水牛及其种牛的 41 个分离物中,MRSA 的毒力基因 clf A、clf B 和 coa 的检出率分别为 100%、80.49% 和 65.85%。目前的研究认为,牛和水牛被认为是 MRSA 的携带者和潜在传播者,这使它们成为人类感染的危险因素,尤其是那些与动物直接接触的人。这些研究结果也突出表明,有必要防止人畜共患病病原体通过职业接触或食用受污染的动物产品传播给人类。
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来源期刊
CiteScore
1.10
自引率
0.00%
发文量
44
审稿时长
5 weeks
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