Optimization of Polyphenols and Antioxidants Extraction from Mentha Suaveolens Subspecies Timija

Abstract

This study delves into the influence of varying solvent compositions on the extraction of total phenolic compounds (TPC) and the antioxidant activities of extracts from Mentha suaveolens subsp. timija leaves and stems. The extraction process is conducted using ultrasound-assisted extraction via an ultrasonic bath. Employing a surface mixture design approach, we explored the singular use of water (W), methanol (M), and ethanol (E), as well as their combinations. The outcomes demonstrate that solvents’ compositions significantly influence the extraction of TPC, with noteworthy synergistic effects observed in specific combinations, such as a ratio of W:M (1/2:1/2). The scavenging activity of DPPH and the total antioxidant capacity (TAC) were also evaluated, indicating that certain combinations of solvents, particularly those involving methanol and water, enhance the antioxidant activities. The results reveal that solvent compositions significantly influence TPC extraction, with notable synergistic effects observed in specific mixtures, such as W:M (1/2:1/2). The Pareto chart analysis highlights the substantial contri - butions of methanol, ethanol, and water to TPC recovery and antioxidant activities. The findings underscore the importance of solvent optimization for extracting bioactive compounds, providing valuable insights for researchers and industries seeking to harness the full potential of this species in various applications.