Hypersensitive detection of CYFRA21-1 by SERS dual antibody sandwich method

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Sensors and Actuators Reports Pub Date : 2024-05-22 DOI:10.1016/j.snr.2024.100198

Song Ge , Mingxin Wang , Shunhua Zhu , Han Wu , Jing Li , Dongsheng Liu , Qingli Huang , Shibao Li , Xiaojing Sun

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Abstract

Purpose

As an important reference index for the early diagnosis of non-small cell lung cancer (NSCLC), CYFRA21-1 still lacks the detection of low equipment cost, wide linear range and high sensitivity. Surface-enhanced Raman scattering (SERS) is a vibration spectrum technology based on the surface plasma of precious metal nanoparticles, which has been effectively applied to the detection of tumor markers. The combination of SERS technology and sensors has great potential in the ultrasensitive detection of tumor markers. The purpose of this study was to develop a sensitive method using SERS to quantitatively detect CYFRA21-1 for early diagnosis of NSCLC.

Methods

A double-antibody sandwich immunoassay based on SERS was designed and tested to implement the ultrasensitive detection of CYFRA21-1 in the serum of NSCLC patients.

Results

Gold @Ag nanorods (Au @Ag NRs) with higher Raman signals were prepared and used as probes, while magnetic graphene oxide was used as a magnetic substrate. The immunized probe, immune substrate and CYFRA21-1 standard substance in the buffer system formed a double-antibody sandwich structure. The standard curve displayed a liner range from 1pg mL⁻¹ to 10 ng mL⁻¹, and the detection limit (LOD) is 0.8943pg mL⁻¹. The Raman intensity exhibited a wide linear relationship with the logarithm of CYFRA21-1 concentration.

Conclusion

Our study successfully established a double-antibody sandwich immunoassay based on SERS. This method demonstrated high specificity and sensitivity for detecting CYFRA21-1 protein content in serum. It has the potential to be applied for early detection of lung cancer biomarkers.

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用 SERS 双抗体夹心法超灵敏检测 CYFRA21-1

目的 CYFRA21-1作为非小细胞肺癌（NSCLC）早期诊断的重要参考指标，目前仍缺乏设备成本低、线性范围宽、灵敏度高的检测手段。表面增强拉曼散射（SERS）是一种基于贵金属纳米颗粒表面等离子体的振动光谱技术，已被有效地应用于肿瘤标志物的检测。SERS 技术与传感器的结合在超灵敏检测肿瘤标志物方面具有巨大潜力。结果制备了具有较高拉曼信号的金@银纳米棒（Au @Ag NRs）作为探针，磁性氧化石墨烯作为磁性基底。免疫探针、免疫底物和 CYFRA21-1 标准物质在缓冲体系中形成双抗体夹心结构。标准曲线的线性范围为 1pg mL-1 至 10 ng mL-1，检出限（LOD）为 0.8943pg mL-1。拉曼强度与 CYFRA21-1 浓度的对数呈宽线性关系。我们的研究成功地建立了一种基于 SERS 的双抗体夹心免疫测定法，该方法在检测血清中 CYFRA21-1 蛋白含量方面具有很高的特异性和灵敏度。它有望应用于肺癌生物标志物的早期检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.