A smartphone-based enhanced colorimetric immunoassay for the detection of Trichinella spiralis infection.

IF 2 2区农林科学 Q2 PARASITOLOGY Veterinary parasitology Pub Date : 2025-01-01 Epub Date: 2024-05-21 DOI:10.1016/j.vetpar.2024.110213

Yao Yu, Lianjing Zhao, Nuo Xu, Xiaolei Liu, Lin Li, Ning Xu, Xue Bai

{"title":"A smartphone-based enhanced colorimetric immunoassay for the detection of Trichinella spiralis infection.","authors":"Yao Yu, Lianjing Zhao, Nuo Xu, Xiaolei Liu, Lin Li, Ning Xu, Xue Bai","doi":"10.1016/j.vetpar.2024.110213","DOIUrl":null,"url":null,"abstract":"<p><p>Trichinellosis is a serious foodborne and zoonotic parasitic disease caused by Trichinella family. At present, the main on-site detection method for Trichinella spiralis (T. spiralis) infection is the lateral flow assay (LFA). Other diagnostic techniques for this parasite cannot be applied to on-site testing due to their reliance on special instruments. Here, we established an ELISA smartphone-based method for detecting anti-T. spiralis antibodies in pig serum. The use of horseradish peroxidase-labeled goat anti-pig IgG-modified gold nanoparticle (AuNPs@HRP-IgG) effectively increased the sensitivity of the method. The entire reaction was carried out at room temperature without the need for special instruments. A low-cost and portable device for imaging and processing experimental data was also developed. Validation analysis revealed that the specificity of the test was 98.89 %, while its sensitivity was 100.00 %. T. spiralis antibodies could be detected in pig serum beginning at 25 dpi after infection with the muscle larvae. This visual immunosensor facilitates on-site detection of T. spiralis, especially in regions lacking specialized laboratory equipment.</p>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":" ","pages":"110213"},"PeriodicalIF":2.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.vetpar.2024.110213","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/21 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Trichinellosis is a serious foodborne and zoonotic parasitic disease caused by Trichinella family. At present, the main on-site detection method for Trichinella spiralis (T. spiralis) infection is the lateral flow assay (LFA). Other diagnostic techniques for this parasite cannot be applied to on-site testing due to their reliance on special instruments. Here, we established an ELISA smartphone-based method for detecting anti-T. spiralis antibodies in pig serum. The use of horseradish peroxidase-labeled goat anti-pig IgG-modified gold nanoparticle (AuNPs@HRP-IgG) effectively increased the sensitivity of the method. The entire reaction was carried out at room temperature without the need for special instruments. A low-cost and portable device for imaging and processing experimental data was also developed. Validation analysis revealed that the specificity of the test was 98.89 %, while its sensitivity was 100.00 %. T. spiralis antibodies could be detected in pig serum beginning at 25 dpi after infection with the muscle larvae. This visual immunosensor facilitates on-site detection of T. spiralis, especially in regions lacking specialized laboratory equipment.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

基于智能手机的增强型比色免疫测定法，用于检测旋毛虫感染。

旋毛虫病是由旋毛虫家族引起的一种严重的食源性和人畜共患寄生虫病。目前，螺旋旋毛虫感染的主要现场检测方法是侧流检测法（LFA）。这种寄生虫的其他诊断技术由于依赖于特殊仪器，无法应用于现场检测。在此，我们建立了一种基于智能手机的 ELISA 方法，用于检测猪血清中的抗螺旋体抗体。辣根过氧化物酶标记的山羊抗猪 IgG修饰金纳米粒子（AuNPs@HRP-IgG）的使用有效提高了该方法的灵敏度。整个反应在室温下进行，无需特殊仪器。此外，还开发了一种用于成像和处理实验数据的低成本便携式设备。验证分析表明，该检测方法的特异性为 98.89%，灵敏度为 100.00%。从肌肉幼虫感染后 25 dpi 开始，猪血清中就能检测到螺旋体抗体。这种视觉免疫传感器有助于现场检测螺旋体，尤其是在缺乏专业实验室设备的地区。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.