High-Quality Complete Genome Sequence of Xanthomonas phaseoli pv. dieffenbachiae Outbreak Strain D182: The Causative Agent of Anthurium Bacterial Blight in Hawai’i

Abstract

Xanthomonas phaseoli pv. dieffenbachiae (Xpd), the causal agent of anthurium blight, is classified as an A2 quarantine organism on the EPPO list due to its devastating impact on the anthurium industry. In this study, we sequenced strain D182, representative of the Hawaiian anthurium blight outbreak (1981-1986), using PacBio RS II SMRT technology. High-quality de novo assembly of 5,217,888 bp (65% GC) was generated with a mean coverage of 351x using HGAP v4. Strain D182 harbors one plasmid (73.5 kb, GC – 60.8%). ANI and dDDH values of 99.86% and 98.9% respectively, showed close phylogenetic relationships with Xpd strain LMG 695PT. The genome information will be useful in providing insights into the genomic biology, virulence mechanisms, and evolutionary relationships of Xpd and other strains associated with anthurium blight outbreaks worldwide.