Genome-wide identification of 9-cis-epoxy-carotenoid dioxygenases (NCEDs) and potential function of OfNCED4 in carotenoid biosynthesis of Osmanthus fragrans

IF 2.1 3区农林科学 Q2 FORESTRY Trees Pub Date : 2024-05-21 DOI:10.1007/s00468-024-02522-9

Yanxia Du, Lin Peng, Bin Dong, Shiwei Zhong, Jinping Deng, Qiu Fang, Zheng Xiao, Yiguang Wang, Hongbo Zhao

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Key message

We identified seven OfNCEDs and analyzed the potential function of OfNCED4, which is involved in the biosynthesis of carotenoids and abscisic acid in Osmanthus fragrans.

Abstract

In plants, 9-cis-epoxycarotenoid dioxygenases (NCEDs) can cleave carotenoids and are rate-limiting enzymes for the biosynthesis of abscisic acid (ABA). Sweet osmanthus (Osmanthus fragrans Lour.), an important ornamental and fragrant tree, contains abundant carotenoids in its flowers. To examine the function of the 9-cis-epoxycarotenoid dioxygenases (NCEDs) involved in ABA biosynthesis and floral coloration in O. fragrans, the sequences of seven OfNCEDs were isolated from the genomic DNA and cDNA of O. fragrans. A bioinformatics analysis showed that these OfNCEDs encode 422 ~ 591 amino acids, and they all contain the RPE65 domain. These OfNCEDs showed different patterns of expression, and that of OfNCED4 was most consistent to the patterns of ABA accumulation during the flowering process. Moreover, the levels of expression of seven OfNCEDs in the O. fragrans flowers were significantly induced by exogenous ABA. ABA-responsive elements were found in the promoters of OfNCEDs. The overexpression of OfNCED4 resulted in increased contents of chlorophylls, carotenoids and ABA, and up-regulation of NtCRTISO, NtLCYE, NtLCYB, and NtNXS, and down-regulation of NtCCD1 and NtCCD4 in tobacco leaves. It suggests that OfNCED4 probably mediates the biosynthesis of ABA. Taken together, this study systematically identified the bioinformatics, pattern of expression of OfNCED genes and the potential function of OfNCED4, which could provide molecular evidence for further research on the regulation of carotenoid biosynthesis and floral color of O. fragrans.

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桂花 9-顺式环氧类胡萝卜素二氧酶（NCED）的全基因组鉴定以及 OfNCED4 在类胡萝卜素生物合成中的潜在功能

摘要在植物中，9-顺式环氧类胡萝卜素二氧酶（NCEDs）可以裂解类胡萝卜素，并且是脱落酸（ABA）生物合成的限速酶。桂花（Osmanthus fragrans Lour.）是一种重要的观赏和芳香树种，其花朵中含有丰富的类胡萝卜素。为了研究参与桂花中 ABA 生物合成和花色的 9-顺式环氧类胡萝卜素二氧酶（NCEDs）的功能，研究人员从桂花的基因组 DNA 和 cDNA 中分离出了 7 个 OfNCEDs 的序列。生物信息学分析表明，这些 OfNCEDs 编码 422 ~ 591 个氨基酸，均含有 RPE65 结构域。这些 OfNCEDs 表现出不同的表达模式，其中 OfNCED4 与开花过程中 ABA 的积累模式最为一致。此外，外源 ABA 能显著诱导七种 OfNCEDs 在芳香木花中的表达水平。在 OfNCEDs 的启动子中发现了 ABA 响应元件。过表达 OfNCED4 会导致烟草叶片中叶绿素、类胡萝卜素和 ABA 含量增加，上调 NtCRTISO、NtLCYE、NtLCYB 和 NtNXS，下调 NtCCD1 和 NtCCD4。这表明 OfNCED4 可能介导了 ABA 的生物合成。综上所述，本研究系统地鉴定了OfNCED基因的生物信息学、表达模式以及OfNCED4的潜在功能，为进一步研究类胡萝卜素生物合成调控和香花花色提供了分子证据。

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来源期刊

Trees 农林科学-林学

CiteScore

4.50

自引率

4.30%

发文量

113

审稿时长

3.8 months

期刊介绍： Trees - Structure and Function publishes original articles on the physiology, biochemistry, functional anatomy, structure and ecology of trees and other woody plants. Also presented are articles concerned with pathology and technological problems, when they contribute to the basic understanding of structure and function of trees. In addition to original articles and short communications, the journal publishes reviews on selected topics concerning the structure and function of trees.