Studies on viability and efficacy of various invigoration techniques to improve germination parameters of Bergenia ciliata (Haw.) Sternb.

Abstract

Bergenia ciliata, a small perennial herb with lithotriptic properties, belongs to the family Saxifragaceae. The rhizome, which is also the most significant commercial part of this plant, is typically used for propagation since the seeds are very tiny with poor viability and germination capacity. Seed invigoration is a well-recognized and effective technique to improve seed germination potential, ensure establishment, and enhance yield under varying environments. Thus, the present investigation aimed to study the seed viability as well as the effect of various invigoration techniques on germination parameters and seedling vigor of B. ciliata. Seeds were primed with 14 distinct conventional treatments varied either with concentrations or duration of exposures. Standardized method was followed for seed germination test, and the emerged seedlings were allowed to grow until day 15 when the various seedling parameters were analyzed. Seed soaking in TZ solution followed by bleaching with 4% NaOCl for 20 min recorded 88.5–95.8% viability. Results also indicated that germination and seedling growth parameters were greater under all priming treatments as compared to control. The maximum germination rate (93.75%) was observed under GA₃@25 ppm. Total seedling length, dry weight and SVI were found to be higher under pre-sowing seed treatment with GA₃@25 ppm in comparison with control or other treatments. Better activation of ‘pre-germinative metabolisms’ in primed seeds might result in improved germination and other indices. Moreover, the PCA and cluster analysis revealed that various pre-sowing treatments particularly GA₃ would be recommended for improved seed germination of B. ciliata.