Developing an Approach for Integrating Chemical Analysis and Transcriptional Changes to Assess Contaminants in Water, Sediment, and Fish

IF 3.6 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Environmental Toxicology and Chemistry Pub Date : 2024-05-27 DOI:10.1002/etc.5886

Ana Sharelys Cardenas Perez, Jonathan K. Challis, Alper James Alcaraz, Xiaowen Ji, Alexis Valerio Valery Ramirez, Markus Hecker, Markus Brinkmann

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Abstract

Pharmaceuticals in aquatic environments pose threats to aquatic organisms because of their continuous release and potential accumulation. Monitoring methods for these contaminants are inadequate, with targeted analyses falling short in assessing water quality's impact on biota. The present study advocates for integrated strategies combining suspect and targeted chemical analyses with molecular biomarker approaches to better understand the risks posed by complex chemical mixtures to nontarget organisms. The research aimed to integrate chemical analysis and transcriptome changes in fathead minnows to prioritize contaminants, assess their effects, and apply this strategy in Wascana Creek, Canada. Analysis revealed higher pharmaceutical concentrations downstream of a wastewater-treatment plant, with clozapine being the most abundant in fathead minnows, showing notable bioavailability from water and sediment sources. Considering the importance of bioaccumulation factor and biota–sediment accumulation factor in risk assessment, these coefficients were calculated based on field data collected during spring, summer, and fall seasons in 2021. Bioaccumulation was classified as very bioaccumulative with values >5000 L kg^–1, suggesting the ability of pharmaceuticals to accumulate in aquatic organisms. The study highlighted the intricate relationship between nutrient availability, water quality, and key pathways affected by pharmaceuticals, personal care products, and rubber components. Prioritization of these chemicals was done through suspect analysis, supported by identifying perturbed pathways (specifically signaling and cellular processes) using transcriptomic analysis in exposed fish. This strategy not only aids in environmental risk assessment but also serves as a practical model for other watersheds, streamlining risk-assessment processes to identify environmental hazards and work toward reducing risks from contaminants of emerging concern. Environ Toxicol Chem 2024;43:2252–2273. © 2024 SETAC

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开发一种综合化学分析和转录变化的方法，以评估水、沉积物和鱼类中的污染物。

水生环境中的药物因其持续释放和潜在积累而对水生生物构成威胁。对这些污染物的监测方法并不完善，有针对性的分析也无法评估水质对生物群的影响。本研究主张采用综合策略，将可疑和目标化学分析与分子生物标记方法相结合，以更好地了解复杂的化学混合物对非目标生物造成的风险。该研究旨在将化学分析与黑头鲦鱼转录组变化相结合，以确定污染物的优先次序、评估其影响，并将这一策略应用于加拿大瓦斯卡纳溪。分析表明，废水处理厂下游的药物浓度较高，其中氯氮平在黑头鲦鱼体内的含量最高，显示了水和沉积物来源的显著生物利用度。考虑到生物累积系数和生物群-沉积物累积系数在风险评估中的重要性，这些系数是根据 2021 年春、夏、秋三个季节收集的实地数据计算得出的。生物蓄积性被归类为生物蓄积性很强，数值大于 5000 升/千克，表明药物能够在水生生物体内蓄积。该研究强调了养分供应、水质以及受药物、个人护理产品和橡胶成分影响的关键途径之间错综复杂的关系。通过对暴露鱼类进行转录组分析，确定受干扰的途径（特别是信号转导和细胞过程），并辅以疑似分析，确定了这些化学物质的优先级。这一策略不仅有助于环境风险评估，还可作为其他流域的实用模型，简化风险评估流程，以确定环境危害并努力降低新出现的污染物所带来的风险。环境毒物化学 2024;00:1-22。© 2024 SETAC.

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来源期刊

Environmental Toxicology and Chemistry 环境科学-毒理学

CiteScore

7.40

自引率

9.80%

发文量

265

审稿时长

3.4 months

期刊介绍： The Society of Environmental Toxicology and Chemistry (SETAC) publishes two journals: Environmental Toxicology and Chemistry (ET&C) and Integrated Environmental Assessment and Management (IEAM). Environmental Toxicology and Chemistry is dedicated to furthering scientific knowledge and disseminating information on environmental toxicology and chemistry, including the application of these sciences to risk assessment.[...] Environmental Toxicology and Chemistry is interdisciplinary in scope and integrates the fields of environmental toxicology; environmental, analytical, and molecular chemistry; ecology; physiology; biochemistry; microbiology; genetics; genomics; environmental engineering; chemical, environmental, and biological modeling; epidemiology; and earth sciences. ET&C seeks to publish papers describing original experimental or theoretical work that significantly advances understanding in the area of environmental toxicology, environmental chemistry and hazard/risk assessment. Emphasis is given to papers that enhance capabilities for the prediction, measurement, and assessment of the fate and effects of chemicals in the environment, rather than simply providing additional data. The scientific impact of papers is judged in terms of the breadth and depth of the findings and the expected influence on existing or future scientific practice. Methodological papers must make clear not only how the work differs from existing practice, but the significance of these differences to the field. Site-based research or monitoring must have regional or global implications beyond the particular site, such as evaluating processes, mechanisms, or theory under a natural environmental setting.

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