Whispers in the Wind: Face Mask Sampling for Mycobacterium tuberculosis Detection in Children With Pulmonary Tuberculosis.

IF 5 2区医学 Q2 IMMUNOLOGY Journal of Infectious Diseases Pub Date : 2024-12-16 DOI:10.1093/infdis/jiae282

Lennard Meiwes, Irina Kontsevaya, Dumitru Chesov, Stela Kulciţkaia, Viola Dreyer, Doris Hillemann, Qiniso Dlamini, Caroline Williams, Michael Barer, Folke Brinkmann, Renate Krüger, Stephanie Thee, Alexander Kay, Anna Maria Mandalakas, Christoph Lange

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Abstract

Background: Recently, face mask sampling (FMS) confirmed detection of Mycobacterium tuberculosis DNA from exhaled breath in adults with tuberculosis. To date, no study has evaluated the use of FMS to detect pulmonary tuberculosis in children. We developed a method for FMS of M. tuberculosis-specific DNA in children and performed a clinical exploration to assess feasibility in children.

Methods: Face masks were spiked, analyzed on GeneXpert-Ultra, quantitative polymerase chain reaction, and targeted next-generation sequencing. Children with pulmonary tuberculosis were asked to wear 3 modified FFP2 masks for 30 minutes as part of an exploratory clinical study.

Results: Experiments with H37Ra M. tuberculosis strain showed a limit of 95% detection of 3.75 colony-forming units (95% confidence interval, 4.85-3.11) on GeneXpert-Ultra. Ten children with pulmonary tuberculosis participated in the clinical study. M. tuberculosis-specific DNA was detected on none of the face masks.

Conclusions: Pediatric FMS has a low limit of detection for M. tuberculosis-specific DNA in vitro. However, M. tuberculosis DNA was not detected in any of 30 masks worn by children with pulmonary tuberculosis. This suggests that FMS in this form may not be more effective for detecting M. tuberculosis in children with tuberculosis than existing methods.

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风中的低语面罩采样用于肺结核患儿的结核分枝杆菌检测。

背景：最近，面罩采样（FMS）证实可从成人肺结核患者呼出的气体中检测出结核分枝杆菌 DNA。迄今为止，还没有研究对使用面罩采样检测儿童肺结核（TB）进行评估。我们开发了一种儿童结核杆菌特异性 DNA 的 FMS 方法，并进行了临床探索，以评估在儿童中的可行性：方法：对口罩进行加标，用 GeneXpert-Ultra、qPCR 和 tNGS 进行分析。作为探索性临床研究的一部分，要求患有肺结核的儿童佩戴三个改良的 FFP2 口罩 30 分钟：结果：使用 H37Ra M. tuberculosis 菌株进行的实验显示，GeneXpert-Ultra 的 95% 检测限为 3.75 CFU (4.85-3.11; 95%CI)。十名肺结核患儿参加了临床研究。所有口罩均未检测到结核杆菌特异性 DNA：结论：儿科 FMS 体外检测结核杆菌特异性 DNA 的限值较低。然而，在肺结核患儿佩戴的 30 个口罩中均未检测到结核杆菌 DNA。这表明，这种形式的 FMS 在检测结核病患儿的结核杆菌方面可能并不比现有方法更有效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infectious Diseases 医学-传染病学

CiteScore

13.50

自引率

3.10%

发文量

449

审稿时长

2-4 weeks

期刊介绍： Published continuously since 1904, The Journal of Infectious Diseases (JID) is the premier global journal for original research on infectious diseases. The editors welcome Major Articles and Brief Reports describing research results on microbiology, immunology, epidemiology, and related disciplines, on the pathogenesis, diagnosis, and treatment of infectious diseases; on the microbes that cause them; and on disorders of host immune responses. JID is an official publication of the Infectious Diseases Society of America.