Potential antivirulence activity of sub-inhibitory concentrations of ciprofloxacin against Proteus mirabilis isolates: an in-vitro and in-vivo study.

IF 4.6 2区医学 Q1 MICROBIOLOGY Annals of Clinical Microbiology and Antimicrobials Pub Date : 2024-05-27 DOI:10.1186/s12941-024-00704-4

Mohamed A Elhosseini, Tarek E El-Banna, Fatma I Sonbol, Maisra M El-Bouseary

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Abstract

Background: Proteus mirabilis is a significant nosocomial pathogen that is frequently associated with a wide range of infections, necessitating heightened attention to mitigate potential health risks. Hence, this study was performed to investigate the impact of sub-minimum inhibitory concentrations (MICs) of ciprofloxacin (CIP) on Proteus mirabilis clinical isolates.

Methods: The sub-MICs of CIP were selected using the growth curve approach. The untreated and treated isolates with sub-MICs of CIP were assessed for their biofilm development, motilities on agar, and other virulence factors. The cell morphology of untreated and treated isolates with sub-MIC of CIP was explored using electron microscope. Moreover, the expression levels of the virulence genes in isolates were measured using quantitative real-time PCR.

Results: Data revealed that sub-MICs of CIP significantly (p < 0.05), in a concentration-dependent manner, inhibited biofilm formation and other virulence factors in the selected isolates. Electron microscope analysis showed cell enlargement and various abnormalities in the cell wall and membrane integrity.

Conclusion: Sub-MICs of CIP exhibited inhibition of virulence and alterations in morphological integrity against P. mirabilis isolates.

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亚抑制浓度环丙沙星对奇异变形杆菌分离株的潜在抗病毒活性：体外和体内研究。

背景：奇异变形杆菌是一种重要的院内病原体，经常与多种感染有关，因此有必要加强关注以降低潜在的健康风险。因此，本研究调查了环丙沙星（CIP）次最低抑菌浓度（MIC）对奇异变形杆菌临床分离株的影响：方法：采用生长曲线法选择 CIP 的亚最低抑菌浓度。方法：采用生长曲线法筛选出 CIP 亚 MICs，并对未处理和处理过的 CIP 亚 MICs 分离物进行生物膜发育、琼脂上的运动和其他毒力因子评估。使用电子显微镜观察了未经处理和经处理的亚微克 CIP 分离物的细胞形态。此外，还利用实时定量 PCR 技术测定了分离物中毒力基因的表达水平：结果：数据显示，CIP 的亚 MIC 显著（P结果：数据显示，CIP 亚微粒对 mirabilis 分离物的毒力和形态完整性的改变具有抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Clinical Microbiology and Antimicrobials MICROBIOLOGY-

CiteScore

8.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.