Gastrodin combined with electroacupuncture prevents the development of cerebral ischemia via rebalance of brain-derived neurotrophic factor and interleukin-6 in stroke model rats.

IF 1.6 4区医学 Q4 NEUROSCIENCES Neuroreport Pub Date : 2024-07-01 Epub Date: 2024-05-16 DOI:10.1097/WNR.0000000000002050

Min Liu, Rujie Gong, Lina Ding, Yingdi Zhao, Xili Yan, Liangbin Shi, Yegui Zhang, Zhiliang Xu

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Abstract

Traditional Chinese medicine (TCM) has long been used to treat various diseases, including cerebral ischemia. The specific molecular mechanism of TCM in the treatment of cerebral ischemia, however, is still unclear. This study investigated the effects of gastrodin, electroacupuncture and their combination on cerebral ischemic rats. We used Nissl staining, immunohistochemical staining and immunoblotting to detect the expression changes of brain-derived neurotrophic factor (BDNF) and interleukin-6 (IL-6) in the frontal cortex. The results showed that the combination therapy of gastrodin and electroacupuncture significantly increased the number of Nissl-positive neurons and improved cell morphology compared with other groups. Mechanistically, we found that the combination of gastrodin and electroacupuncture treatment group can restore the abnormal morphology of neuronal cells caused by cerebral ischemia by rebalancing the expression levels of BDNF and IL-6. Our research indicates that gastrodin combined with electroacupuncture has a significant protective effect on cerebral ischemic injury in rats, possibly by regulating the expression of BDNF and IL-6. This combination therapy is superior to single-drug or electroacupuncture therapy.

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天麻素联合电针通过重新平衡脑源性神经营养因子和白细胞介素-6防止中风模型大鼠脑缺血的发生。

长期以来，中医一直被用于治疗包括脑缺血在内的各种疾病。然而，中医药治疗脑缺血的具体分子机制尚不清楚。本研究探讨了天麻素、电针及其组合对脑缺血大鼠的影响。我们采用Nissl染色法、免疫组织化学染色法和免疫印迹法检测了脑源性神经营养因子（BDNF）和白细胞介素-6（IL-6）在额叶皮层的表达变化。结果显示，与其他组相比，胃复安和电针联合治疗能显著增加Nissl阳性神经元的数量，并改善细胞形态。从机理上讲，我们发现天麻素联合电针治疗组可以通过重新平衡BDNF和IL-6的表达水平来恢复脑缺血导致的神经元细胞的异常形态。我们的研究表明，天麻素联合电针对大鼠脑缺血损伤有显著的保护作用，可能是通过调节BDNF和IL-6的表达。这种联合疗法优于单一药物或电针疗法。

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来源期刊

Neuroreport 医学-神经科学

CiteScore

3.20

自引率

0.00%

发文量

150

审稿时长

1 months

期刊介绍： NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.