Synthesis of Zwitterionic Phospholipid-Connected Silane Coupling Agents and Their Hybridization with Graphene Oxide

IF 1.6 4区农林科学 Q3 CHEMISTRY, APPLIED Journal of oleo science Pub Date : 2024-06-01 DOI:10.5650/jos.ess24044

Kanato Arita, Seina Yarimizu, Maiko Moriguchi, Tsuyoshi Inoue, Haruyasu Asahara

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Abstract

The hybridization of lipids with graphene is expected to produce a promising, novel biomaterial. However, there are limited examples of the covalent introduction of lipid molecules, especially the immobilization of lipid molecules, onto graphene on a substrate. Therefore, we investigated the hybridization of a silane coupling agent having phospholipid moieties with graphene oxide on substrates prepared by photo-oxidation using chlorine dioxide. Three silane coupling agents with different carbon chain lengths (C4, C6, C8) were synthesized and phospholipid molecules were introduced onto graphene on a substrate. Phospholipid-immobilized graphene on a grid for TEM (transmission electron microscope) was used for EM analysis of proteins (glyceraldehyde 3-phosphate dehydrogenase and β-galactosidase), enabling the observation of sufficient particles compared to the conventional graphene grid.

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合成与石墨烯氧化物杂化的硅烷偶联剂及与磷脂连接的硅烷偶联剂

脂质与石墨烯的杂化有望产生一种前景广阔的新型生物材料。然而，将脂质分子共价引入基底石墨烯，特别是将脂质分子固定在石墨烯上的例子非常有限。因此，我们研究了具有磷脂分子的硅烷偶联剂与氧化石墨烯在二氧化氯光氧化制备的基底上的杂化。我们合成了三种不同碳链长度（C4、C6、C8）的硅烷偶联剂，并将磷脂分子引入基底上的石墨烯。将磷脂固定在 TEM（透射电子显微镜）网格上的石墨烯用于蛋白质（3-磷酸甘油醛脱氢酶和 β-半乳糖苷酶）的电磁分析，与传统的石墨烯网格相比，可以观察到足够多的颗粒。

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来源期刊

Journal of oleo science CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

3.20

自引率

6.70%

发文量

173

审稿时长

3 months

期刊介绍： The J. Oleo Sci. publishes original researches of high quality on chemistry, biochemistry and science of fats and oils such as related food products, detergents, natural products, petroleum products, lipids and related proteins and sugars. The Journal also encourages papers on chemistry and/or biochemistry as a major component combined with biological/ sensory/nutritional/toxicological evaluation related to agriculture and/or food.