The nearly complete genome sequence of clade B, wheat streak mosaic virus isolate from Türkiye

Abstract

Wheat streak mosaic virus is one of the most widespread viruses in cereal crops, causing severe losses and dramatically affecting worldwide wheat production. Currently, four distinct clades of WSMV have been grouped and named: A (Mexico), B (Europe, Asia, Russia, the USA), C (Iran), and D (The USA, Argentina, Brazil, Australia, Canada, Türkiye). In this study, we determined the nearly complete and partial nucleotide sequences of WSMV type B isolates collected from wheat found in the European part of Türkiye using high throughput sequencing. Excluding the poly(A) tail, the genome of isolate S34Edirne (GenBank acc.no. MZ405098) consisted of 9360 nucleotides and contained a single large open reading frame encoding a polyprotein of 3033 amino acids. The partial sequence of WSMV in wheat isolate (GenBank acc.no. ON364116) is 3348 nt long. The characteristic lack of a GGA (Gly₂₇₆₁) codon within coat protein (CP) of the polyprotein of S34Edirne complete nucleotide sequence is typical for the clade B, WSMV-ΔE isolates, which are widely found throughout the European continent. Sequence comparisons revealed that WSMV Turkish wheat isolate is the most closely related to Czech isolate, with highly similar nucleotide and amino acid identities at 98.83 and 99.13%, respectively. The result of this study indicates that the nearly complete and partial genome sequences of S34Edirne and S34_Edirne24 isolates should be placed into clade B of the European WSMV-ΔE isolates.