Genetic Polymorphism of GH and IGF-1 Genes and Body Measurements Traits in Maghrabi Camel

IF 0.5 4区生物学 Q4 BIOLOGY Biology Bulletin Pub Date : 2024-05-31 DOI:10.1134/s1062359024606712

A. M. Darwish, M. A. Abdelhafez, S. I. Othman, H. A. El-Metwaly, H. A. Rudayni, A. A. Allam

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Abstract

Nonetheless the key roles of growth hormone (GH) and insulin growth factor-1 (IGF-1) genes in livestock growth traits (including body measurements), few studies deployed them in camels, unlike other livestock species (cattle, buffalo, sheep, and goat). So, this study was performed to investigate the genetic polymorphism of GH and IGF-1 markers, in Maghrabi camels by PCR-SSCP and DNA sequencing tools, with assessing the impacted live body measurements traits. In this study, 65 blood samples were collected from Maghrabi camel, and different body measurements were valued. Different genotypes of GH and IGF-1 genes were determined, via SSCP-PCR and sequencing tools. The results pointed out one genotype for the IGF-I gene, and three genotypes for the GH gene, with 23.21, 42.85, and 33.95 frequencies, of AA, AB, and BB genotypes, respectively. The sequence analysis showed specific single nucleotide polymorphisms (SNPs), for both A and B alleles. Those SNPs triggered alteration in some of the amino acids. Moreover, individuals of the BB genotype recorded the highest value in body weight, Neck length, Height at lumbar area, Anterior limb length, Neck circumference, and increased Body length associated with the AA genotype. Nevertheless, there were no significant differences, between the three genotypes, for the other body measurements. This study found that Maghrabi camels possess three genotypes of the GH gene and one genotype of the IGFI gene, and the BB genotype of the GH gene was associated with high performance of growth traits. Therefore, it is useful to use camel individuals, with the BB genotype of the GH gene, in camelid breeding programs, to improve live body measurement traits.

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马格里布骆驼 GH 和 IGF-1 基因的遗传多态性与体型特征

摘要尽管生长激素（GH）和胰岛素生长因子-1（IGF-1）基因在家畜生长性状（包括体型测量）中发挥着关键作用，但与其他家畜物种（牛、水牛、绵羊和山羊）不同的是，很少有研究在骆驼中使用这些基因。因此，本研究通过 PCR-SSCP 和 DNA 测序工具研究马格拉比骆驼 GH 和 IGF-1 标记的遗传多态性，并评估其对活体测量特征的影响。本研究收集了 65 份马格拉比骆驼的血液样本，并对不同的身体测量值进行了评估。通过 SSCP-PCR 和测序工具测定了 GH 和 IGF-1 基因的不同基因型。结果显示，IGF-I 基因有一种基因型，GH 基因有三种基因型，AA、AB 和 BB 基因型的频率分别为 23.21、42.85 和 33.95。序列分析显示，A 和 B 等位基因存在特定的单核苷酸多态性（SNPs）。这些 SNP 引发了部分氨基酸的改变。此外，与 AA 基因型相比，BB 基因型个体的体重、颈长、腰围、前肢长、颈围和体长的数值最高。然而，三种基因型之间在其他身体测量指标上没有明显差异。本研究发现，马格拉比骆驼拥有三种 GH 基因基因型和一种 IGFI 基因基因型，其中 GH 基因的 BB 基因型与高生长性能相关。因此，在骆驼育种计划中使用具有 GH 基因 BB 基因型的骆驼个体对改善活体测量性状很有帮助。

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来源期刊

Biology Bulletin 生物-生物学

CiteScore

0.70

自引率

20.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Biology Bulletin (Izvestiya Rossiiskoi Akademii Nauk – Seriya Biologicheskaya) is an interdisciplinary journal of general biology. It focuses on fundamental studies in the fields of cell biology, biochemistry, zoology, botany, physiology, and ecology. This journal publishes current materials of experimental studies and surveys on current problems in general biology. It also publishes information on scientific conferences and new books in the fields of general biology.