New Haplotypes of Blastocystis sp. Identified in Faeces from Various Animal Groups in Algeria

IF 1.2 3区农林科学 Q4 PARASITOLOGY Acta Parasitologica Pub Date : 2024-06-03 DOI:10.1007/s11686-024-00857-4

Asma Guilane, Ikram Haleche, Fadila Tazerouti, Hocine Ziam, Tahar Kernif, Amina Boutellis

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Abstract

Purpose

Blastocystis sp. is a single-celled, anaerobic, parasitic protozoan commonly found in the intestinal tract of animals and humans globally. Genetic analysis has revealed significant diversity within its species, leading to the identification of at least 40 subtypes (ST1–ST40). This study aimed to identify and differentiate Blastocystis in faeces samples from various animal hosts in Algeria.

Methods

A total of 403 fecal samples, collected from both domestic and zoo animals, were subjected to PCR amplification and sequencing of Blastocystis-specific small subunit ribosomal RNA (SSU-RNA) gene.

Results

The overall prevalence of Blastocystis in animals was found to be 38.9%. Through comprehensive phylogenetic and phylogeographic analyses, we identified four distinct subtypes (ST1 in both domestic and zoo animals, and ST3, ST4, and ST5 exclusively in zoo animals), encompassing nine different haplotypes, including five that appear original to Algeria.

Conclusion

This study represents the first epidemiological molecular investigation of Blastocystis sp. in animals in Algeria.

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从阿尔及利亚不同动物群体的粪便中鉴定出 Blastocystis sp.

目的：布氏囊尾蚴（Blastocystis sp.）是一种单细胞厌氧寄生原生动物，常见于全球动物和人类的肠道中。遗传分析表明，该物种内部存在显著的多样性，从而确定了至少 40 个亚型（ST1-ST40）。本研究旨在鉴定和区分阿尔及利亚各种动物宿主粪便样本中的布氏囊虫：方法：对从家养动物和动物园收集的 403 份粪便样本进行 PCR 扩增，并对 Blastocystis 特异性小亚基核糖体 RNA（SSU-RNA）基因进行测序：结果表明：动物中大疱菌的总体流行率为 38.9%。通过全面的系统发生学和系统地理学分析，我们确定了四种不同的亚型（ST1在家畜和动物园动物中均有发生，ST3、ST4和ST5仅在动物园动物中发生），包括九种不同的单倍型，其中五种似乎是阿尔及利亚独有的单倍型：本研究是阿尔及利亚首次对动物中的布氏囊虫进行流行病学分子调查。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.