Immunofluorescent Identification of Dystrophin, Actin, and Light and Heavy Myosin Chains in Somatic Cells of Earthworm Lumbricus terrestris

Q4 Biochemistry, Genetics and Molecular Biology Cell and Tissue Biology Pub Date : 2024-06-03 DOI:10.1134/s1990519x24700287
L. F. Nurullin, E. M. Volkov
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Abstract

In muscle cells of the motor muscle of earthworm Lumbricus terrestris, the dystrophin and actin proteins and fast and slow isoforms of myosin heavy chains were determined using fluorescence microscopy. It can be thought that the expression of these proteins occurred at the earliest stages of the evolutionary formation of the intracellular contractile apparatus of the motor tissue in both invertebrates and vertebrates. This study will complement the picture of the evolutionary development of motor muscle tissue.

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蚯蚓 Lumbricus terrestris 体细胞中肌萎缩蛋白、肌动蛋白、轻肌球蛋白链和重肌球蛋白链的免疫荧光鉴定
摘要 利用荧光显微镜测定了蚯蚓运动肌的肌肉细胞中肌萎缩蛋白和肌动蛋白以及肌球蛋白重链的快慢异构体。可以认为,这些蛋白质的表达发生在无脊椎动物和脊椎动物运动组织胞内收缩装置进化形成的最早阶段。这项研究将补充运动肌肉组织进化发展的图景。
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来源期刊
Cell and Tissue Biology
Cell and Tissue Biology Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
0.80
自引率
0.00%
发文量
51
期刊介绍: The journal publishes papers on vast aspects of cell research, including morphology, biochemistry, biophysics, genetics, molecular biology, immunology. The journal accepts original experimental studies, theoretical articles suggesting novel principles and approaches, presentations of new hypotheses, reviews highlighting major developments in cell biology, discussions. The main objective of the journal is to provide a competent representation and integration of research made on cells (animal and plant cells, both in vivo and in cell culture) offering insight into the structure and functions of live cells as a whole. Characteristically, the journal publishes articles on biology of free-living and parasitic protists, which, unlike Metazoa, are eukaryotic organisms at the cellular level of organization.
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