Clinical significance of ribosomal protein S15 expression in patients with colorectal cancer liver metastases

IF 3.2 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Journal of Hepato‐Biliary‐Pancreatic Sciences Pub Date : 2024-06-04 DOI:10.1002/jhbp.12012

Yoshihiro Sakano, Daijiro Matoba, Takehiro Noda, Shogo Kobayashi, Daisaku Yamada, Yoshito Tomimaru, Hidenori Takahashi, Mamoru Uemura, Yuichiro Doki, Hidetoshi Eguchi

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Abstract

Background

Liver metastasis is the most frequently observed distant metastasis of colorectal cancer, and the residual liver recurrence rate after hepatic resection is still high. To explore the mechanism of liver metastasis to discover potential new treatments, we assessed the relationship between the expression of differentially expressed genes (DEGs) and prognosis in patients with colorectal cancer liver metastasis (CRLM).

Methods

The gene expression dataset was extracted from The Cancer Genome Atlas and the Gene Expression Omnibus. Significance analysis of DEGs between tumor and normal samples of colorectum, liver, and lung was conducted. A total of 80 CRLM patients were studied to assess the expression of RPS15, characteristics, and outcomes. We examined the relationships of RPS15 expression to cell viability and apoptosis in vitro and vivo.

Results

Significance analysis identified 33 DEGs. In our cohorts, the overall survival rates were significantly lower in the high-RPS15-expression group, and high expression of RPS15 was an independent and unfavorable prognostic factor in recurrence-free survival and overall survival. Knockdown of RPS15 expression reduced the proliferative capacity of colorectal cancer cells and increased BAX-induced apoptotic cell death.

Conclusions

RPS15 expression is an independent prognostic factor for CRLM patients and might be a novel therapeutic target for CRLM.

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结直肠癌肝转移患者核糖体蛋白 S15 表达的临床意义。

背景：肝转移是结直肠癌最常见的远处转移灶，肝切除术后的残肝复发率仍然很高。为了探索肝转移的机制以发现潜在的新疗法，我们评估了结直肠癌肝转移（CRLM）患者中差异表达基因（DEGs）的表达与预后之间的关系：基因表达数据集来自癌症基因组图谱（The Cancer Genome Atlas）和基因表达总库（Gene Expression Omnibus）。方法：从癌症基因组图谱（The Cancer Genome Atlas）和基因表达总库（Gene Expression Omnibus）中提取基因表达数据集，对肿瘤和结直肠、肝脏、肺部正常样本之间的 DEGs 进行显著性分析。共研究了 80 例 CRLM 患者，以评估 RPS15 的表达、特征和预后。我们研究了RPS15的表达与体外和体内细胞活力和凋亡的关系：结果：显著性分析确定了 33 个 DEGs。在我们的队列中，RPS15高表达组的总生存率明显较低，RPS15的高表达是无复发生存率和总生存率的一个独立且不利的预后因素。敲除RPS15表达可降低结直肠癌细胞的增殖能力，增加BAX诱导的细胞凋亡：RPS15的表达是CRLM患者的一个独立预后因素，可能是CRLM的一个新的治疗靶点。

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来源期刊

Journal of Hepato‐Biliary‐Pancreatic Sciences GASTROENTEROLOGY & HEPATOLOGY-SURGERY

自引率

10.00%

发文量

178

审稿时长

6-12 weeks

期刊介绍： The Journal of Hepato-Biliary-Pancreatic Sciences (JHBPS) is the leading peer-reviewed journal in the field of hepato-biliary-pancreatic sciences. JHBPS publishes articles dealing with clinical research as well as translational research on all aspects of this field. Coverage includes Original Article, Review Article, Images of Interest, Rapid Communication and an announcement section. Letters to the Editor and comments on the journal’s policies or content are also included. JHBPS welcomes submissions from surgeons, physicians, endoscopists, radiologists, oncologists, and pathologists.