Relationship between P16INK4a Overexpression in Human Papillomavirus Infection in High Grade CIN and Cervical Carcinoma

Abstract

Background: High-risk HPV strains are a well-known contributor to the carcinogenesis of cervical squamous cell carcinoma (SCC). Indeed, there has been a high detection of HPV DNA in SCC and precancerous lesions. Objective: The aim of the study was to assess the Relationship Between Human Papillomavirus Infection and P16INK4a Overexpression in Cervical Squamous Cell Carcinoma. Methodology: This descriptive cross-sectional study was carried out in the Pathology Department of DMC with collaboration of Bangabandhu Sreikh Mujib Medical University (BSMMU), Microbiology Department and Bangladesh Agricultural University (BAU), Mymansingh, during July 2017 to June 2018. A total 50 VIA positive and clinically suspected premalignant and malignant patient’s attendant at DMC included as study population. Colposcopic biopsy specimens were sent to the Department of Pathology, DMCH, for histopathological examination. Some portion of biopsy samples were preserved at -4ºC for HR-HPV DNA detection. Statistical analyses were done by using the SPSS 26.0. Result: Highest number of cases 20 (40%) were in fourth decade and their mean age was found to be 44.20±11.20, ranging from 28 to 67 years. Among 50 positive cases, maximum number of 32 (62%) cases were married between 16 to 20 years of age, 12 (22%) cases were married in less than 15 years of age and 6 (8%) cases were married at more than 20 years of age. The findings of colposcopic examinations of total 50 cases. Common diagnoses were CIN II in 24 (48%) cases, 8(16%) case represented CIN III, Squamous cell carcinoma in 16 (32%) cases and Adeno Carcinoma 2(4%) cases. In comparison of p16 score with cervical lesions in study subjects there were 26 true positive cases and 13 true negative cases. According to p16 score a total 37 (26 true positive+ 11 false positive) cases were p16 positive. Out of which 16(32.0%) and 21(42.0%) were HR-HPV positive and negative respectively. Similarly according to p16 score 13 nevative cases ........