Effects of sample storage temperature and duration on the detection of foliar endophytes of tea plants (Camellia sinensis L.) in summer and winter.

IF 2.2 4区生物学 Q3 MICROBIOLOGY Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae035

Phyu Mar Win, Norihisa Matsushita, Kenji Fukuda

引用次数: 0

Abstract

Seasonal changes in the diversity of tea plant endophytic fungi and the effects of sample storage conditions on detection were analyzed. Tea leaves were collected from the Saitama Tea Research Institute in Japan during winter (January 2020) and summer (August 2020). The effects of storage temperature (5, 10, 20, 25, and 30°C) and durations (1, 2, 3, 4, 5, 6, and 7 days) on endophytic fungal diversity and community structure were investigated. In summer, storage period and temperature did not affect the fungal colonization rate, frequency, and composition. In winter, storage temperature and period significantly affected the endophytic community structure. Fungal diversity was higher in winter than in summer. Positive relationships between diversity index and storage temperature and period were observed in winter, whereas the opposite trend was observed in summer. Our findings provide insight into the ecology of foliar endophytes of tea plants and the importance of proper sample collection and storage for microbiome studies.

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样品储存温度和时间对夏季和冬季茶树（Camellia sinensis L.）叶片内生菌检测的影响。

分析了茶树内生真菌多样性的季节性变化以及样品储存条件对检测的影响。茶叶分别于冬季（2020 年 1 月）和夏季（2020 年 8 月）从日本埼玉茶叶研究所采集。研究了贮藏温度（5 °C、10 °C、20 °C、25 °C和 30 °C）和贮藏时间（1、2、3、4、5、6 和 7 天）对内生真菌多样性和群落结构的影响。在夏季，贮藏期和温度对真菌的定植率、频率和组成没有影响。在冬季，储存温度和时间对内生真菌群落结构有明显影响。冬季的真菌多样性高于夏季。冬季的多样性指数与储藏温度和储藏期呈正相关，而夏季则相反。我们的研究结果有助于深入了解茶树叶片内生菌的生态学，以及正确采集和储存样本对微生物组研究的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.