Analysis of T follicular and T peripheral helper lymphocytes in autoimmune thyroid disease.

IF 3 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Endocrine Pub Date : 2024-11-01 DOI:10.1007/s12020-024-03686-7

Raquel Sánchez-Gutiérrez, Rebeca Martínez-Hernández, Ana Serrano-Somavilla, Miguel Sampedro-Nuñez, Alejandra Mendoza-Pérez, José Luis Muñoz de Nova, Marlen Vitales-Noyola, Roberto González-Amaro, Mónica Marazuela

{"title":"Analysis of T follicular and T peripheral helper lymphocytes in autoimmune thyroid disease.","authors":"Raquel Sánchez-Gutiérrez, Rebeca Martínez-Hernández, Ana Serrano-Somavilla, Miguel Sampedro-Nuñez, Alejandra Mendoza-Pérez, José Luis Muñoz de Nova, Marlen Vitales-Noyola, Roberto González-Amaro, Mónica Marazuela","doi":"10.1007/s12020-024-03686-7","DOIUrl":null,"url":null,"abstract":"Purpose: Peripheral helper T (Tph) cells have an important role in the induction of humoral immune responses and autoantibody production. Accordingly, it is feasible that this lymphocyte subset has a relevant role in the pathogenesis of autoimmune thyroid diseases (AITD). In this study we aim to analyze the levels and function of Tph cells in blood samples from patients with AITD.Methods: We performed an observational study with cases and controls. Blood samples were obtained from nineteen patients with Hashimoto's thyroiditis (HT), twenty-four with Graves' disease (GD), and fifteen healthy controls. In addition, the levels of follicular T helper (Tfh) cells and Tph cells, the release of interleukin-21 (IL-21) by these lymphocytes and the number of plasmablasts were analyzed by multi-parametric flow cytometry analyses.Results: Increased percentages of Tfh and Tph lymphocytes were detected in patients with HT and GD. Furthermore, an enhanced synthesis of the cytokine IL-21 by these cells was observed. Accordingly, we detected significant higher percentages of plasmablasts in patients with GD, and these values tended to be also higher in HT patients. Moreover, significant positive associations were observed between the levels of Tfh or Tph and the number of plasmablast or anti-TSHR Ab titers in patients with AITD.Conclusion: Our data suggest that Tph lymphocytes may have a relevant role in the pathogenesis of AITD.","PeriodicalId":49211,"journal":{"name":"Endocrine","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11489195/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endocrine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s12020-024-03686-7","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: Peripheral helper T (Tph) cells have an important role in the induction of humoral immune responses and autoantibody production. Accordingly, it is feasible that this lymphocyte subset has a relevant role in the pathogenesis of autoimmune thyroid diseases (AITD). In this study we aim to analyze the levels and function of Tph cells in blood samples from patients with AITD.

Methods: We performed an observational study with cases and controls. Blood samples were obtained from nineteen patients with Hashimoto's thyroiditis (HT), twenty-four with Graves' disease (GD), and fifteen healthy controls. In addition, the levels of follicular T helper (Tfh) cells and Tph cells, the release of interleukin-21 (IL-21) by these lymphocytes and the number of plasmablasts were analyzed by multi-parametric flow cytometry analyses.

Results: Increased percentages of Tfh and Tph lymphocytes were detected in patients with HT and GD. Furthermore, an enhanced synthesis of the cytokine IL-21 by these cells was observed. Accordingly, we detected significant higher percentages of plasmablasts in patients with GD, and these values tended to be also higher in HT patients. Moreover, significant positive associations were observed between the levels of Tfh or Tph and the number of plasmablast or anti-TSHR Ab titers in patients with AITD.

Conclusion: Our data suggest that Tph lymphocytes may have a relevant role in the pathogenesis of AITD.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

自身免疫性甲状腺疾病中T滤泡和T外周辅助淋巴细胞的分析。

目的：外周辅助性 T（Tph）细胞在诱导体液免疫反应和自身抗体产生方面起着重要作用。因此，该淋巴细胞亚群可能在自身免疫性甲状腺疾病（AITD）的发病机制中扮演重要角色。本研究旨在分析 AITD 患者血液样本中 Tph 细胞的水平和功能：我们对病例和对照组进行了观察研究。我们采集了 19 名桥本氏甲状腺炎（HT）患者、24 名巴塞杜氏病（GD）患者和 15 名健康对照者的血液样本。此外，还通过多参数流式细胞术分析了滤泡T辅助细胞（Tfh）和Tph细胞的水平、这些淋巴细胞释放的白细胞介素-21（IL-21）以及浆细胞的数量：结果：在 HT 和 GD 患者中检测到 Tfh 和 Tph 淋巴细胞百分比增加。此外，还观察到这些细胞合成细胞因子 IL-21 的能力增强。相应地，我们在 GD 患者中检测到明显较高比例的浆细胞，这些数值在 HT 患者中也有较高的趋势。此外，在 AITD 患者中还观察到 Tfh 或 Tph 水平与浆细胞数量或抗 TSHR Ab 滴度之间存在明显的正相关：我们的数据表明，Tph 淋巴细胞可能在 AITD 的发病机制中起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Endocrine ENDOCRINOLOGY & METABOLISM-

CiteScore

6.50

自引率

5.40%

发文量

295

审稿时长

1.5 months

期刊介绍： Well-established as a major journal in today’s rapidly advancing experimental and clinical research areas, Endocrine publishes original articles devoted to basic (including molecular, cellular and physiological studies), translational and clinical research in all the different fields of endocrinology and metabolism. Articles will be accepted based on peer-reviews, priority, and editorial decision. Invited reviews, mini-reviews and viewpoints on relevant pathophysiological and clinical topics, as well as Editorials on articles appearing in the Journal, are published. Unsolicited Editorials will be evaluated by the editorial team. Outcomes of scientific meetings, as well as guidelines and position statements, may be submitted. The Journal also considers special feature articles in the field of endocrine genetics and epigenetics, as well as articles devoted to novel methods and techniques in endocrinology. Endocrine covers controversial, clinical endocrine issues. Meta-analyses on endocrine and metabolic topics are also accepted. Descriptions of single clinical cases and/or small patients studies are not published unless of exceptional interest. However, reports of novel imaging studies and endocrine side effects in single patients may be considered. Research letters and letters to the editor related or unrelated to recently published articles can be submitted. Endocrine covers leading topics in endocrinology such as neuroendocrinology, pituitary and hypothalamic peptides, thyroid physiological and clinical aspects, bone and mineral metabolism and osteoporosis, obesity, lipid and energy metabolism and food intake control, insulin, Type 1 and Type 2 diabetes, hormones of male and female reproduction, adrenal diseases pediatric and geriatric endocrinology, endocrine hypertension and endocrine oncology.