Enantioanalysis of Leucine and Arginine: A Key Factor in Lung Cancer Metabolomics

Abstract

Metabolomics has become a key factor for identifying new biomarkers able to be used for early diagnosis of cancer. A zinc oxide-α-cyclodextrin nanocarbon nanocomposite 3D stochastic microsensor (α-CD/ZnO/nanoC) was designed and integrated into an intelligent 3D stochastic enantioselective platform for the enantioanalysis of arginine and leucine in whole blood samples. The α-CD/ZnO/nanoC pastes were morphologicaly characterized by scanning electron microscopy, and electrochemically characterized by cyclic voltammetry and electrochemical impedance spectroscopy. L-Leucine was determined on a working concentration range between 110-11-110-8mol L-1 with a limit of detection of 310-12mol L-1; D-leucine was determined on a working concentration range between 110-16-110-11mol L-1 with a limit of detection of 310-17mol L-1; L-arginine was determined on a working concentration range between 110-12-110-9mol L-1 with a limit of detection of 310-13mol L-1; D-arginine was determined on a working concentration range between 110-14-110-11mol L-1 with a limit of detection of 310-15mol L-1. High sensitivities, selectivities, and enantioselectivities were recorded when the platform was used for the enantioanalysis of arginine and leucine. High recoveries were recorded at the enantioanalysis of arginine/leucine, with low relative standard deviations.