A Sorption–Spectrometric Method for Quantitation of Catecholamines in Urine and Plasma Using Hypercrosslinked Polystyrene and Gold Nanorods or Their Nanocomposites

Abstract

The possibility of combining solid-phase extraction of natural catecholamines (adrenaline, norepinephrine, and dopamine) on a microcolumn filled with hypercrosslinked polystyrene with their subsequent spectrophotometric or solid-phase spectroscopic determination in an eluate using gold nanorods or their nanocomposites with polyurethane foam has been investigated. The desorption of catecholamines from hypercrosslinked polystyrene by various eluents has been studied. It has been shown that the quantitative elution of all three catecholamines is achieved using 6 M acetic acid, which as an eluent is well combined with the proposed determination options. The features of a dynamic sorption extraction of catecholamines from urine and blood serum have been described. It allows you to reduce the influence of the matrix components and increase the analysis sensitivity by concentrating. The average concentration coefficient is 10–56 with sample volumes of 10–300 mL. The developed methods make it possible to determine catecholamines in urine at the level of their normal content.