Optimization and kinetic study of the bioethanol production by a locally isolated strain using response surface methodology

Abstract

This study explores the use of a locally isolated yeast strain to enhance bioethanol production. The research investigates the impact of various parameters on bioethanol and glycerol production using the Algerian isolated yeast strain “Saccharomyces cerevisiae” in conjunction with sugarcane byproducts. Fermentation kinetic parameters were determined, and optimization was carried out using response surface methodology (RSM), focusing on sugar concentration (measured in Brix degrees) and inoculum volume (%). Logistic and adapted Gompertz kinetics models were utilized to analyze fermentation duration against bioethanol output. RSM identified optimal conditions with an inoculum size of 6.6% and 25°Bx. Kinetic parameters derived from the model include a peak ethanol concentration (P_m) of 73.55 g/L, a maximum ethanol generation rate (r_p,m) of 2.61 g/L/h, and a latency phase duration (t_L) of 1.47 h. The study showcases the significant potential of the locally isolated yeast strain in achieving substantial bioethanol yields from sugarcane molasses, paving the way for large-scale production efforts.