{"title":"Cell type-specific control and post-translational regulation of specialized metabolism: opening new avenues for plant metabolic engineering","authors":"Nikolaos Ntelkis , Alain Goossens , Krešimir Šola","doi":"10.1016/j.pbi.2024.102575","DOIUrl":null,"url":null,"abstract":"<div><p>Although plant metabolic engineering enables the sustainable production of valuable metabolites with many applications, we still lack a good understanding of many multi-layered regulatory networks that govern metabolic pathways at the metabolite, protein, transcriptional and cellular level. As transcriptional regulation is better understood and often reviewed, here we highlight recent advances in the cell type-specific and post-translational regulation of plant specialized metabolism. With the advent of single-cell technologies, we are now able to characterize metabolites and their transcriptional regulators at the cellular level, which can refine our searches for missing biosynthetic enzymes and cell type-specific regulators. Post-translational regulation through enzyme inhibition, protein phosphorylation and ubiquitination are clearly evident in specialized metabolism regulation, but not frequently studied or considered in metabolic engineering efforts. Finally, we contemplate how advances in cell type-specific and post-translational regulation can be applied in metabolic engineering efforts <em>in planta</em>, leading to optimization of plants as metabolite production vehicles.</p></div>","PeriodicalId":11003,"journal":{"name":"Current opinion in plant biology","volume":null,"pages":null},"PeriodicalIF":8.3000,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current opinion in plant biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369526624000669","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Although plant metabolic engineering enables the sustainable production of valuable metabolites with many applications, we still lack a good understanding of many multi-layered regulatory networks that govern metabolic pathways at the metabolite, protein, transcriptional and cellular level. As transcriptional regulation is better understood and often reviewed, here we highlight recent advances in the cell type-specific and post-translational regulation of plant specialized metabolism. With the advent of single-cell technologies, we are now able to characterize metabolites and their transcriptional regulators at the cellular level, which can refine our searches for missing biosynthetic enzymes and cell type-specific regulators. Post-translational regulation through enzyme inhibition, protein phosphorylation and ubiquitination are clearly evident in specialized metabolism regulation, but not frequently studied or considered in metabolic engineering efforts. Finally, we contemplate how advances in cell type-specific and post-translational regulation can be applied in metabolic engineering efforts in planta, leading to optimization of plants as metabolite production vehicles.
期刊介绍:
Current Opinion in Plant Biology builds on Elsevier's reputation for excellence in scientific publishing and long-standing commitment to communicating high quality reproducible research. It is part of the Current Opinion and Research (CO+RE) suite of journals. All CO+RE journals leverage the Current Opinion legacy - of editorial excellence, high-impact, and global reach - to ensure they are a widely read resource that is integral to scientists' workflow.