Comprehensive Immunohistochemical Analysis of Epithelial-Mesenchymal Transition Biomarkers in the Invasive Micropapillary Cancer of the Breast.

IF 1.6 Q4 ONCOLOGY International Journal of Breast Cancer Pub Date : 2024-06-11 eCollection Date: 2024-01-01 DOI:10.1155/2024/2350073
Ozden Oz, Funda Alkan Tasli, Resmiye Irmak Yuzuguldu, Baha Zengel, Demet Kocatepe Cavdar, Merih Guray Durak, Raika Durusoy
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Abstract

Background: Invasive micropapillary carcinoma (IMPC) of the breast is commonly associated with a poor prognosis due to its high incidence of lymphovascular invasion and lymph node metastasis (LNM). Our study is aimed at investigating the prognostic significance of the expressions of E-cadherin (E-cad), N-cadherin (N-cad), CD44s, and β-catenin (β-cat). In addition, it is aimed at deciphering the consistency of these markers between the IMPC, the invasive breast carcinoma, no-special type (IBC-NST), and LNM components in the same IMPC cases. Methods: Sixty-two IMPC cases with LNM from 1996 to 2018 were analyzed. Immunohistochemical staining was performed separately on the three regions for each patient. Statistical analyses included Kaplan-Meier, Cox regression, and McNemar's statistical tests. Results: Loss of CD44 expression in IMPC, IBC-NST, and LNM areas was associated with poor prognosis in overall survival (OS) (p = 0.010, p < 0.0005, p = 0.025). Loss of CD44 expression in the IBC-NST, gain of N-cad expression in the IMPC, and loss of β-cat expression in the LNM areas were indicators of poor prognosis in disease-free survival (DFS) (p = 0.005, p = 0.041, p = 0.009). Conclusion: Our evaluation of this rare subtype, focusing on the expression of key epithelial-mesenchymal transition (EMT) molecules, revealed that it shares characteristics with the IBC-NST component within mixed tumors. Notably, contrary to expectations, a reduction in CD44 expression was found to adversely affect both OS and DFS. By conducting staining procedures simultaneously across three regions within the same patient, a novel approach has provided valuable insights into the mechanisms of EMT.

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乳腺浸润性微乳头状癌上皮-间质转化生物标志物的免疫组化综合分析
背景:乳腺浸润性微乳头状癌(IMPC)由于淋巴管侵犯和淋巴结转移(LNM)发生率高,通常预后较差。我们的研究旨在探讨 E-cad、N-cad、CD44s 和 β-catenin(β-cat)表达的预后意义。此外,该研究还旨在解读同一 IMPC 病例中 IMPC、浸润性乳腺癌、无特殊类型(IBC-NST)和 LNM 成分之间这些标记物的一致性。研究方法分析了1996年至2018年期间62例伴有LNM的IMPC病例。对每位患者的三个区域分别进行免疫组化染色。统计分析包括 Kaplan-Meier、Cox 回归和 McNemar 统计检验。结果CD44在IMPC、IBC-NST和LNM区域的表达缺失与总生存期(OS)的不良预后相关(p = 0.010,p < 0.0005,p = 0.025)。IBC-NST中CD44表达缺失、IMPC中N-cad表达增高、LNM区域中β-cat表达缺失是无病生存期(DFS)预后不良的指标(p = 0.005、p = 0.041、p = 0.009)。结论我们对这一罕见亚型进行了评估,重点研究了关键上皮-间质转化(EMT)分子的表达,结果发现它与混合瘤中的IBC-NST成分具有相同的特征。值得注意的是,与预期相反,CD44表达的减少会对OS和DFS产生不利影响。通过对同一患者的三个区域同时进行染色,一种新方法为了解 EMT 的机制提供了宝贵的见解。
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来源期刊
CiteScore
3.40
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: International Journal of Breast Cancer is a peer-reviewed, Open Access journal that provides a forum for scientists, clinicians, and health care professionals working in breast cancer research and management. The journal publishes original research articles, review articles, and clinical studies related to molecular pathology, genomics, genetic predisposition, screening and diagnosis, disease markers, drug sensitivity and resistance, as well as novel therapies, with a specific focus on molecular targeted agents and immune therapies.
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