Characterization and Pathogenicity of a Porcine Reproductive and Respiratory Syndrome Virus Strain with Strong Homology to a HP-PRRSV Vaccine Strain in the Field

IF 3.5 2区农林科学 Q2 INFECTIOUS DISEASES Transboundary and Emerging Diseases Pub Date : 2024-06-21 DOI:10.1155/2024/1297975

Chunhua Wei, Xin Lan, Wenlin Huang, Yuan Yang, Hui Yu, Chen Liu, Cuiqin Huang, Manlin Luo, Jiankui Liu

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Abstract

A PRRSV strain, PRRSV2/CN/FJLX06/2021, with strong homology to an MLV-like strain HeN1201 that evolved from the highly pathogenic PRRSV vaccine virus HuN4-F112, was isolated from a dying piglet in China. BLAST and phylogenetic analyses showed that PRRSV2/CN/FJLX06-2021 was most closely related to HeN1201 and HuN4 (the parental strain of MLV HuN4-F112) and clustered with Chinese HP-PRRSV strains in PRRSV-2 lineage 8.7. Importantly, 29 of the 39 characteristic amino acid mutations in the HuN4-F112 genome were found at the corresponding sites of PRRSV2/CN/FJLX06-2021. Animal studies showed that piglets infected with PRRSV2/CN/FJLX06-2021 had a persistent high fever, higher viremia, presence of interstitial pneumonia, and a higher mortality rate (40%) within 2 weeks than those vaccine-inoculated with HuN4-F112. Taken together, these data suggest that PRRSV2/CN/FJLX06-2021 is an MLV-like strain that has evolved from MLV HuN4-F112 and is highly pathogenic to piglets.

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猪繁殖与呼吸综合征病毒株的特征和致病性与 HP-PRRSV 疫苗株在田间具有很强的同源性

从中国一头濒临死亡的仔猪身上分离出了 PRRSV 毒株 PRRSV2/CN/FJLX06/2021，该毒株与从高致病性 PRRSV 疫苗病毒 HuN4-F112 演化而来的 MLV 样毒株 HeN1201 有很强的同源性。BLAST 和系统进化分析表明，PRRSV2/CN/FJLX06-2021 与 HeN1201 和 HuN4（MLV HuN4-F112 的亲本株）的亲缘关系最密切，并与中国的 HP-PRRSV 株系聚类在 PRRSV-2 8.7 系中。重要的是，在 HuN4-F112 基因组的 39 个特征氨基酸突变中，有 29 个突变出现在 PRRSV2/CN/FJLX06-2021 的相应位点上。动物实验表明，与接种 HuN4-F112 疫苗的仔猪相比，感染 PRRSV2/CN/FJLX06-2021 的仔猪在 2 周内会出现持续高热、病毒血症、间质性肺炎和更高的死亡率（40%）。综上所述，这些数据表明 PRRSV2/CN/FJLX06-2021 是由 MLV HuN4-F112 演化而来的 MLV 样毒株，对仔猪具有高致病性。

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来源期刊

Transboundary and Emerging Diseases 农林科学-传染病学

CiteScore

8.90

自引率

9.30%

发文量

350

审稿时长

1 months

期刊介绍： Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.