Comparison of Mir122 expression in children with biliary atresia and healthy group.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biology Research Communications Pub Date : 2024-01-01 DOI:10.22099/mbrc.2024.49649.1950
Nasrin Motazedian, Negar Azarpira, Kimia Falamarzi, Seyed Mohsen Dehghani, Maryam Ataollahi, Elaheh Esfandiari, Mahintaj Dara, Razieh Toobafard, Mehrab Sayadi, Seyed Ali Shekarforoush, Seyed Hossein Owji, Seyed Ali Malekhosseini
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Abstract

Biliary atresia (BA) is the primary cause of neonatal jaundice with various pathological mechanisms. Many BA patients may experience progressive liver dysfunction and eventually need a liver transplant. Therefore, identifying potential non-invasive biomarkers for BA is crucial. miR-122, the most abundant microRNA in the liver, plays significant roles in different liver diseases. This study aimed to assess miR-122 levels in BA patients. Eighteen patients with biliary atresia were selected at random from the Shiraz Pediatric Liver Cirrhosis Cohort Study (SPLCCS), along with 18 healthy controls. Blood samples were collected, and biochemical parameters (such as liver function tests) were measured. Quantitative reverse-transcription PCR (RT-PCR) was conducted on serum samples from both the case and control groups to analyze miR-122 levels. The study results indicated that serum miR-122 expression in BA patients was elevated compared to the control group, although it did not reach statistical significance. Additionally, no correlation was found between miR-122 expression and serum levels of liver enzymes or other laboratory findings in BA cases. miR-122 could be a potential target for diagnosing BA; however, further research with a larger population is necessary to determine if miR-122 could serve as a useful biomarker for diagnosing BA.

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胆道闭锁儿童与健康组 Mir122 表达的比较
胆道闭锁(BA)是导致新生儿黄疸的主要原因,其病理机制多种多样。许多胆道闭锁患者会出现进行性肝功能障碍,最终需要进行肝移植。miR-122是肝脏中含量最高的微RNA,在不同的肝脏疾病中发挥着重要作用。本研究旨在评估 BA 患者体内的 miR-122 水平。研究人员从设拉子小儿肝硬化队列研究(SPLCCS)中随机抽取了18名胆道闭锁患者和18名健康对照者。采集血样并测量生化指标(如肝功能检测)。研究人员对病例组和对照组的血清样本进行了定量反转录 PCR(RT-PCR),以分析 miR-122 的水平。研究结果表明,与对照组相比,BA 患者血清中的 miR-122 表达升高,但未达到统计学意义。然而,要确定 miR-122 能否作为诊断 BA 的有用生物标志物,还需要对更多人群进行进一步研究。
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来源期刊
Molecular Biology Research Communications
Molecular Biology Research Communications BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
3.00
自引率
0.00%
发文量
12
期刊介绍: “Molecular Biology Research Communications” (MBRC) is an international journal of Molecular Biology. It is published quarterly by Shiraz University (Iran). The MBRC is a fully peer-reviewed journal. The journal welcomes submission of Original articles, Short communications, Invited review articles, and Letters to the Editor which meets the general criteria of significance and scientific excellence in all fields of “Molecular Biology”.
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