Telomerase activity, telomere length, and the euploidy rate of human embryos.

IF 2 4区 医学 Q3 ENDOCRINOLOGY & METABOLISM Gynecological Endocrinology Pub Date : 2024-12-01 Epub Date: 2024-07-01 DOI:10.1080/09513590.2024.2373742
Maria Longo, Ermanno Greco, Ilaria Listorti, Maria Teresa Varricchio, Katerina Litwicka, Cristiana Arrivi, Cecilia Mencacci, Pierfrancesco Greco
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Abstract

Background: Telomeres maintain chromosome stability, while telomerase counteracts their progressive shortening. Telomere length varies between cell types, with leukocyte telomere length (LTL) decreasing with age. Reduced telomerase activity has been linked to reproductive issues in females, such as low pregnancy rates and premature ovarian failure, with recent studies indicating correlations between telomere length in granulosa cells and IVF outcomes.

Objectives: The study aims to explore the relationship between telomere length, telomerase activity, and euploid blastocyst rate in infertile women undergoing IVF/ICSI PGT-A cycles.

Methods: This prospective study involves 108 patients undergoing controlled ovarian stimulation and PGT-A. Telomere length and telomerase activity were measured in peripheral mononuclear cells and granulosa cells (GC), respectively.

Results: The telomere repeat copy number to single gene copy number ratio (T/S) results respectively 0.6 ± 0.8 in leukocytes and 0.7 ± 0.9 in GC. An inverse relationship was found between LTL and the patient's age (p < .01). A higher aneuploid rate was noticed in patients with short LTL, with no differences in ovarian reserve markers (p = .15), number of oocytes retrieved (p = .33), and number of MII (p = 0.42). No significant association was noticed between telomere length in GC and patients' age (p = 0.95), in ovarian reserve markers (p = 0.32), number of oocytes retrieved (p = .58), number of MII (p = .74) and aneuploidy rate (p = .65).

Conclusion: LTL shows a significant inverse correlation with patient age and higher aneuploidy rates. Telomere length in GCs does not correlate with patient age or reproductive outcomes, indicating differential telomere dynamics between leukocytes and granulosa cells.

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端粒酶活性、端粒长度和人类胚胎的非整倍体率。
背景端粒能维持染色体的稳定性,而端粒酶则能抵消端粒的逐渐缩短。端粒长度因细胞类型而异,白细胞端粒长度(LTL)随年龄增长而减少。端粒酶活性降低与女性生殖问题有关,如怀孕率低和卵巢早衰,最近的研究表明颗粒细胞端粒长度与试管婴儿结果之间存在相关性:本研究旨在探讨接受IVF/ICSI PGT-A周期治疗的不孕女性的端粒长度、端粒酶活性和优倍囊胚率之间的关系:这项前瞻性研究涉及 108 名接受控制性卵巢刺激和 PGT-A 的患者。方法:这项前瞻性研究涉及108名接受控制性卵巢刺激和PGT-A的患者,分别测量了外周单核细胞和颗粒细胞(GC)的端粒长度和端粒酶活性:结果:端粒重复拷贝数与单基因拷贝数之比(T/S)分别为:白细胞 0.6 ± 0.8,粒细胞 0.7 ± 0.9。发现LTL与患者年龄(p = .15)、取卵数量(p = .33)和MII数量(p = 0.42)呈反向关系。GC的端粒长度与患者的年龄(p = 0.95)、卵巢储备指标(p = 0.32)、取卵细胞数(p = .58)、MII数(p = .74)和非整倍体率(p = .65)均无明显关联:结论:LTL与患者年龄和较高的非整倍体率呈明显的反相关。GC的端粒长度与患者年龄或生殖结果无关,这表明白细胞和颗粒细胞的端粒动态存在差异。
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来源期刊
Gynecological Endocrinology
Gynecological Endocrinology 医学-妇产科学
CiteScore
4.40
自引率
5.00%
发文量
137
审稿时长
3-6 weeks
期刊介绍: Gynecological Endocrinology , the official journal of the International Society of Gynecological Endocrinology, covers all the experimental, clinical and therapeutic aspects of this ever more important discipline. It includes, amongst others, papers relating to the control and function of the different endocrine glands in females, the effects of reproductive events on the endocrine system, and the consequences of endocrine disorders on reproduction
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