Annexin A5 is a novel prognostic biomarker in oral squamous cell carcinoma

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-06-30 DOI:10.1111/jop.13567

Ting Zhou, Xiaoxin Zhang, Yuxian Song, Liang Ding, Xiaofeng Huang, Lei Zhang, Chuanjin Ye, Yan Yang, Antonio Celentano, Qingang Hu, Yanhong Ni

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Abstract

Background

ANXA5, a notable tumor marker, displays irregular expression in diverse solid cancers, and links to local recurrence and metastasis rates. We aimed study the expression of ANXA5 in oral squamous cell carcinoma (OSCC) and its diagnostic and prognostic values.

Methods

520 head and neck squamous cell carcinoma (HNSCC) patients in TCGA database and 124 OSCC patients in Nanjing stomatology hospital were enrolled in our study. Immunohistochemical analyses were performed using ANXA5 antibodies. Chi-square test was used to analyze the clinicopathological features. Survival rates were determined using the Kaplan–Meier method and log-rank test.

Results

Our results showed significantly elevated ANXA5 at the gene and protein levels in HNSCC and OSCC compared to non-tumor tissues. Histopathologically, ANXA5 was broadly present in OSCC tumor cells and fibroblast-like cells but absent in tumor-infiltrating lymphocytes, particularly at the invasive tumor front. Patients exhibiting high ANXA5 expression in these cells demonstrated poor differentiation, aggressive invasion patterns, and heightened lymph node metastasis risk, contributing to poorer postoperative outcomes. Remarkably, ANXA5 in fibroblast-like cells emerged as an independent risk factor impacting survival in OSCC patients. Gene set enrichment analysis (GSEA) highlighted ANXA5's involvement in key pathways like epithelial-mesenchymal transformation (EMT), TGF-beta signaling, and hypoxia, which correlated with adverse clinical outcomes in OSCC.

Conclusion

ANXA5 emerges as a significant prognostic biomarker for OSCC, potentially influencing its metastasis via the EMT pathway.

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Annexin A5 是口腔鳞状细胞癌的一种新型预后生物标志物。

背景：ANXA5是一种显著的肿瘤标志物，在多种实体瘤中均有不规则表达，并与局部复发率和转移率有关。我们旨在研究ANXA5在口腔鳞状细胞癌（OSCC）中的表达及其诊断和预后价值。方法：我们纳入了TCGA数据库中的520例头颈部鳞状细胞癌（HNSCC）患者和南京市口腔医院的124例OSCC患者。使用ANXA5抗体进行免疫组化分析。采用卡方检验分析临床病理特征。采用Kaplan-Meier法和对数秩检验确定生存率：结果表明，与非肿瘤组织相比，HNSCC和OSCC中ANXA5的基因和蛋白水平明显升高。从组织病理学角度看，ANXA5广泛存在于OSCC肿瘤细胞和成纤维细胞样细胞中，但在肿瘤浸润淋巴细胞中却不存在，尤其是在肿瘤浸润前沿。这些细胞中ANXA5高表达的患者分化不良，侵袭模式具有侵袭性，淋巴结转移风险增加，导致术后效果较差。值得注意的是，成纤维细胞样细胞中的ANXA5是影响OSCC患者生存的独立风险因素。基因组富集分析（GSEA）强调了ANXA5参与上皮-间质转化（EMT）、TGF-beta信号转导和缺氧等关键通路，而这些通路与OSCC的不良临床结局相关：结论：ANXA5是OSCC的重要预后生物标志物，可能通过EMT途径影响其转移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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