Kanamycin treated-ethylenediamine tetraacetic acid-dependent pseudothrombocytopenia in blood specimen of cats.

IF 0.9 Q3 VETERINARY SCIENCES Open Veterinary Journal Pub Date : 2024-05-01 Epub Date: 2024-05-31 DOI:10.5455/OVJ.2024.v14.i5.15

Narissara Keawchana, Pirayu Rakwong, Pratthana Yongsakulchai

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Abstract

Background: Pseudothrombocytopenia is a commonly obtained false negative result when analyzing feline platelet (PLT) count by an automated machine. It is related to ethylenediamine tetra-acetic acid (EDTA), a widely utilized anticoagulant in blood collection tubes, resulting in EDTA-dependent pseudothrombocytopenia (EDTA-PTCP).

Aim: To investigate whether treated with kanamycin enhanced the quantity of PLT aggregations in feline blood specimens collected using EDTA-PTCP.

Methods: Thirty-one blood samples were obtained using EDTA tubes. The complete blood count was analyzed using an automated Mindray BC-5000Vet. Both Manual cell counts and thin blood smears were performed to estimate the amount of red blood cell, white blood cell, and PLTs as well as to evaluate the severity scores of PLT clumping, respectively. Comparisons were made between those pre-treated and those treated with kanamycin in the EDTA tube.

Results: There were significantly different mean PLT counts in the samples before and after they were treated with kanamycin, both on automated (156.6 ± 76.4 vs. 260.3 ± 115.5; p < 0.001) and manual (168.5 ± 92.1 vs. 262.8 ± 119.6; p < 0.001) readings, with a 95% confidence interval of 0.19 (0.022-0.365).

Conclusion: This study suggests that in clinical laboratory practice, kanamycin should be added to feline blood specimens with EDTA-PTCP.

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经卡那霉素处理的猫血液标本中乙二胺四乙酸依赖性假性血小板减少症。

背景：假性血小板减少症是用自动机器分析猫科动物血小板（PLT）计数时常见的假阴性结果。它与采血管中广泛使用的抗凝剂乙二胺四乙酸（EDTA）有关，导致 EDTA 依赖性假性血小板减少症（EDTA-PTCP）。目的：研究使用卡那霉素是否会增强使用 EDTA-PTCP 采集的猫科动物血液样本中 PLT 的聚集数量：方法：使用 EDTA 管采集 31 份血液样本。方法：使用 EDTA 管采集了 31 份血液样本，并使用全自动 Mindray BC-5000Vet 分析了全血细胞计数。人工细胞计数和薄层血涂片分别用于估算红细胞、白细胞和 PLT 的数量，以及评估 PLT 结块的严重程度。对预先处理和在 EDTA 管中使用卡那霉素处理的患者进行比较：结果：样本在使用卡那霉素处理前后的平均 PLT 计数有明显差异，无论是自动读数（156.6 ± 76.4 vs. 260.3 ± 115.5；p < 0.001）还是手动读数（168.5 ± 92.1 vs. 262.8 ± 119.6；p < 0.001），95% 置信区间均为 0.19 (0.022-0.365)：本研究表明，在临床实验室实践中，应在猫科动物血液标本中添加卡那霉素与 EDTA-PTCP。

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.