In Vitro Studies of Pumpkin (Cucurbita moschata var. Kashi Harit) Seed Protein Fraction(s) to Evaluate Anticancer and Antidiabetic Properties.

IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-09-01 Epub Date: 2024-07-01 DOI:10.1007/s11130-024-01205-7
S Vinayashree, C Hemakumar, Ravindra P Veeranna, Ravi Kumar, V Pavithra, V P Mahendra, Prasanna Vasu
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Abstract

Chronic diseases like cancer and diabetes are the major public health concerns of India and worldwide. Nowadays, plant-derived products are in great demand for the treatment of these diseases. Pumpkin seeds are traditionally implicated for their pharmacological properties, as exemplified by benign prostatic hyperplasia. Earlier, pumpkin seed proteins were extracted by the Osborne method, and their functional and nutritional qualities were evaluated. Here, the aim is to assess in vitro, the anticancer and antidiabetic properties of seed protein fractions. HepG2, MDA-MB-231, and MCF-7 cell lines were treated with water-soluble (WF) and alkali-soluble fractions (AF) to assess cytotoxicity, while pancreatic β-cells and insulin resistance (IR) - HepG2 cell lines were treated with WF to evaluate the antidiabetic potential. WF and AF showed cytotoxic effects towards HepG2 and MDA-MB-231 cell lines, suggesting apoptosis-mediated anticancerous activity. WF potentiates glucose-stimulated insulin secretion in pancreatic β-cells, in a dose-dependent manner. In IR-HepG2 cell line studies, control, metformin, and WF-treated groups showed uptake of glucose, when compared to the diabetic group, which is well-correlated with the upregulated expressions of GLUT2 and GLUT4 transporters in these groups. These results indicate that proteins from WF and AF may have anticancerous and antidiabetic properties and thus have the potential to utilize pumpkin proteins in the management of cancer and diabetes.

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评估南瓜(Cucurbita moschata var.
癌症和糖尿病等慢性疾病是印度乃至全球关注的主要公共卫生问题。如今,治疗这些疾病的植物提取物需求量很大。南瓜籽历来被认为具有药理特性,良性前列腺增生就是一例。早些时候,人们用奥斯本法提取了南瓜籽蛋白质,并对其功能和营养品质进行了评估。本文旨在体外评估南瓜籽蛋白质组分的抗癌和抗糖尿病特性。用水溶性(WF)和碱溶性(AF)馏分处理 HepG2、MDA-MB-231 和 MCF-7 细胞系,以评估细胞毒性;用 WF 处理胰岛β细胞和胰岛素抵抗(IR)- HepG2 细胞系,以评估抗糖尿病潜力。WF 和 AF 对 HepG2 和 MDA-MB-231 细胞株有细胞毒性作用,表明它们具有凋亡介导的抗癌活性。WF 能以剂量依赖的方式增强葡萄糖刺激的胰腺 β 细胞的胰岛素分泌。在IR-HepG2细胞系研究中,与糖尿病组相比,对照组、二甲双胍组和WF处理组都显示出对葡萄糖的吸收,这与这些组中GLUT2和GLUT4转运体的表达上调有很好的相关性。这些结果表明,WF 和 AF 的蛋白质可能具有抗癌和抗糖尿病的特性,因此有可能将南瓜蛋白质用于癌症和糖尿病的治疗。
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来源期刊
Plant Foods for Human Nutrition
Plant Foods for Human Nutrition 工程技术-食品科技
CiteScore
6.80
自引率
7.50%
发文量
89
审稿时长
12-24 weeks
期刊介绍: Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods
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