Bioactivity responses to changes in mucus-associated bacterial composition between healthy and bleached Porites lobata corals

IF 3.6 3区 生物学 Q1 ZOOLOGY Journal of invertebrate pathology Pub Date : 2024-07-01 DOI:10.1016/j.jip.2024.108164
Van Ngoc Bui , Thi Phuong Thao Nguyen , Huy Duong Nguyen , Quyet Tien Phi , Trung Nam Nguyen , Hoang Ha Chu
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Abstract

This study aims to investigate how bioactivities of the coral surface mucus layer (SML) respond to changes in mucus-associated bacterial communities between bleached and healthy Porites lobata corals in Nha Trang Bay, Vietnam. The findings suggested that significant shifts in the mucus-associated bacterial communities were related to changes in coral health states from bleached to healthy P. lobata colonies (p < 0.05), while bacterial compositions were not significantly different across seasons and locations (p > 0.05). Of which 8 genera, Shewanella, Fusibacter, Halodesulfovibrio, Marinifilum, Endozoicomonas, Litoribacillus, Algicola, and Vibrio were present only in the SML of bleached coral while absent in the SML of the healthy one. As compared with the bleached SML, the healthy SML demonstrated stronger antibacterial activity against a coral bleaching pathogen, V. coralliilyticus, higher antitumor activity against HCT116 cell accompanied with increased induction of cleaved PARP and accelerated cell nucleic apoptosis and cycle arrest at S and G2/M phases exhibiting several typical characteristics, cell shrinkage, lost cell contact, and apoptotic body formation. Moreover, putative compounds detected at 280 nm in the healthy SML were obviously higher than those in the bleached one, probably they could be bioactive molecules responsible for competitively exclusion of pathogens, Algicola and Vibrio, from the healthy SML.

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健康珊瑚与白化珊瑚之间的粘液相关细菌组成变化的生物活性反应。
本研究旨在探讨珊瑚表面粘液层(SML)的生物活性如何响应越南芽庄湾白化珊瑚与健康龙虾珊瑚之间粘液相关细菌群落的变化。研究结果表明,粘液相关细菌群落的显著变化与珊瑚健康状况从白化到健康的变化有关(p 0.05)。其中有 8 个菌属,即 Shewanella、Fusibacter、Halodesulfovibrio、Marinifilum、Endozoicomonas、Litoribacillus、Algicola 和 Vibrio 只存在于白化珊瑚的 SML 中,而在健康珊瑚的 SML 中则不存在。与白化的 SML 相比,健康的 SML 对珊瑚白化病原体 V. coralliilyticus 具有更强的抗菌活性,对 HCT116 细胞具有更高的抗肿瘤活性,同时还能诱导更多的 PARP 分解,加速细胞核凋亡,使细胞周期停滞在 S 期和 G2/M 期,表现出几个典型特征:细胞萎缩、细胞接触消失和凋亡体形成。此外,在 280 纳米波长下检测到的健康 SML 中的推定化合物明显高于漂白 SML 中的推定化合物,这可能是健康 SML 中竞争性排斥病原体(藻类和弧菌)的生物活性分子。
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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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