A. A. Semenova, A. D. Igoshkina, N. V. Mikina, R. G. Savchenko, L. V. Parfenova, M. V. Dubinin
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引用次数: 0
Abstract
In this work we have studied the effect of the phytoecdysteroid 20-hydroxyecdysone (20E) on the functioning of mouse skeletal muscle mitochondria. It is shown that 20E at a concentration of 100 µM or more suppresses mitochondrial respiration fueled by glutamate and malate (substrates of complex I of the respiratory chain) or succinate (substrate of complex II of the respiratory chain). This effect of 20E is accompanied by a decrease in the mitochondrial membrane potential and is associated with inhibition of the activity of complex III, the total activity of complexes I + III and II + III of the mitochondrial respiratory chain. We have noted a prooxidant effect of 20E, which manifests itself in an increase in the production of hydrogen peroxide by skeletal muscle mitochondria. In addition, 20E reduces the ability of mitochondria to accumulate calcium ions in the matrix. We discuss the mechanisms of the possible toxic effect of 20E on the functioning of skeletal muscle mitochondria.
摘要 在这项工作中，我们研究了植物蜕皮激素 20-羟基蜕皮激素（20E）对小鼠骨骼肌线粒体功能的影响。研究表明，浓度为 100 µM 或更高的 20E 可抑制以谷氨酸和苹果酸（呼吸链复合体 I 的底物）或琥珀酸（呼吸链复合体 II 的底物）为燃料的线粒体呼吸。20E 的这种作用伴随着线粒体膜电位的降低，并与抑制复合体 III 的活性、线粒体呼吸链复合体 I + III 和复合体 II + III 的总活性有关。我们注意到 20E 具有促氧化作用，表现为骨骼肌线粒体产生的过氧化氢增加。此外，20E 还会降低线粒体在基质中积累钙离子的能力。我们讨论了 20E 对骨骼肌线粒体功能可能产生毒性影响的机制。
期刊介绍：
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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