Knockdown of GNL3 inhibits LUAD cell growth by regulating Wnt-β-catenin pathway.

IF 2.5 4区医学 Q3 ALLERGY Allergologia et immunopathologia Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI:10.15586/aei.v52i4.1117

Guihong Dai, Yuejun Sun

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Abstract

Background: Lung adenocarcinoma (LUAD) is a leading cause of tumor-associated mortality, and it is needed to find new target to combat this disease. Guanine nucleotide-binding -protein-like 3 (GNL3) mediates cell proliferation and apoptosis in several cancers, but its role in LUAD remains unclear.

Objective: To explore the expression and function of Guanine nucleotide-binding protein-like 3 (GNL3) in lung adenocarcinoma (LUAD) and its potential mechanism in inhibiting the growth of LUAD cells.

Methods: We evaluated the expression of GNL3 in LUAD tissues and its association with patient prognosis using databases and immunohistochemistry. Cell proliferation was assessed by CCK-8 assay as well as colony formation, while apoptosis was evaluated by FCM. The effect of GNL3 knockdown on the Wnt/β-catenin axis was investigated by Immunoblot analysis.

Results: GNL3 is overexpressed in LUAD tissues and is correlated with poor prognosis. Knockdown of GNL3 significantly inhibited the growth as well as induced apoptosis in A549 as well as H1299 cells. Furthermore, we found that the inhibitory effect of GNL3 knockdown on LUAD cell growth is associated with the downregulation of the Wnt/β-catenin axis.

Conclusion: GNL3 is key in the progression of LUAD by metiating Wnt/β-catenin axis. Targeting GNL3 may represent a novel therapeutic method for LUAD treatment.

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敲除 GNL3 可通过调节 Wnt-β-catenin 通路抑制 LUAD 细胞的生长。

背景：肺腺癌（LUAD）是导致肿瘤相关死亡的主要原因之一，因此需要找到新的靶点来对抗这种疾病。鸟嘌呤核苷酸结合蛋白样 3（GNL3）在多种癌症中介导细胞增殖和凋亡，但其在肺腺癌中的作用仍不清楚：目的：探讨类鸟嘌呤核苷酸结合蛋白 3（GNL3）在肺腺癌（LUAD）中的表达和功能及其抑制 LUAD 细胞生长的潜在机制：我们使用数据库和免疫组化方法评估了 GNL3 在 LUAD 组织中的表达及其与患者预后的关系。细胞增殖通过 CCK-8 试验和集落形成进行评估，细胞凋亡通过 FCM 进行评估。免疫印迹分析研究了GNL3基因敲除对Wnt/β-catenin轴的影响：结果：GNL3在LUAD组织中过表达，并与不良预后相关。敲除 GNL3 能显著抑制 A549 和 H1299 细胞的生长并诱导其凋亡。此外，我们还发现敲除 GNL3 对 LUAD 细胞生长的抑制作用与 Wnt/β-catenin 轴的下调有关：结论：通过调控Wnt/β-catenin轴，GNL3是LUAD进展的关键。靶向 GNL3 可能是治疗 LUAD 的一种新方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.