Chloroquine regulates the lipopolysaccharide-induced inflammatory response in RAW264.7 cells.

IF 2.5 4区医学 Q3 ALLERGY Allergologia et immunopathologia Pub Date : 2024-07-01 eCollection Date: 2024-01-01 DOI:10.15586/aei.v52i4.1083

Natsuki Ota, Shoya Endo, Kouki Honma, Kuninori Iwayama, Hiroshi Yamashita, Ryosuke Tatsunami, Keisuke Sato

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Abstract

Introduction and objectives: Macrophage-induced inflammation plays a key role in defense against injury and harmful pathogens. Autophagy and the inflammatory response are associated; however, the relationship between the autophagy pathway and lipopolysaccharide (LPS)- induced inflammatory responses remains unknown. We aimed to determine the effect of autophagy on the LPS-induced myeloid differentiation factor 88 (MyD88)/nuclear transcription factor kB (NF-kB) pathway-mediated inflammatory response in RAW264.7 cells.

Materials and methods: To determine the effect of autophagy on the LPS-induced inflammatory response, using various in vitro assays, we determined the effect of autophagy inhibitors and inducers on the inflammatory response in RAW264.7 cells.

Results: Chloroquine (CQ), an autophagy inhibitor, suppressed pro-inflammatory cytokines, including interleukin (IL)-1β, IL-6, and tumor necrosis factor α (TNFα) in LPS-stimulated RAW264.7 cells. CQ also affected inflammatory mediators such as myeloid differentiation factor 88 and NF-kB in LPS-stimulated RAW264.7 cells.

Conclusion: This study demonstrated that CQ regulates the LPS-induced inflammatory response in RAW264.7 cells. We propose that targeting the regulation of pro-inflammatory cytokine levels and inflammatory mediators using CQ is a promising therapeutic approach for preventing inflammatory injury. CQ serves as a potential therapeutic target for treating various inflammatory diseases.

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氯喹调节脂多糖诱导的 RAW264.7 细胞炎症反应。

引言和目的：巨噬细胞诱导的炎症在抵御损伤和有害病原体方面发挥着关键作用。自噬与炎症反应相关；然而，自噬途径与脂多糖（LPS）诱导的炎症反应之间的关系仍然未知。我们旨在确定自噬对 RAW264.7 细胞中 LPS 诱导的髓系分化因子 88（MyD88）/核转录因子 kB（NF-kB）通路介导的炎症反应的影响：为了确定自噬对LPS诱导的炎症反应的影响，我们利用各种体外试验，确定了自噬抑制剂和诱导剂对RAW264.7细胞炎症反应的影响：结果：自噬抑制剂氯喹（CQ）抑制了 LPS 刺激的 RAW264.7 细胞中的促炎细胞因子，包括白细胞介素（IL）-1β、IL-6 和肿瘤坏死因子α（TNFα）。CQ还影响了LPS刺激的RAW264.7细胞中的炎症介质，如髓系分化因子88和NF-kB：本研究表明，CQ 可调节 LPS 诱导的 RAW264.7 细胞炎症反应。我们认为，利用 CQ 靶向调节促炎细胞因子水平和炎症介质是一种很有前景的预防炎症损伤的治疗方法。CQ 是治疗各种炎症性疾病的潜在治疗靶点。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.