The Protective Effect of Juçara Fruit (Euterpe edulis Martius) Extracts on LPS-Activated J774 Macrophages.

IF 3.1 2区农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-09-01 Epub Date: 2024-07-08 DOI:10.1007/s11130-024-01204-8

Mayara Schulz, Luciano Valdemiro Gonzaga, Ana Clara Nascimento Antunes, Tainá Lubschinski, Eduarda Talita Bramorski Mohr, Eduardo Monguilhott Dalmarco, Carolina Turnes Pasini Deolindo, Rodrigo Barcellos Hoff, Fábio Martinho Zambonim, Ana Carolina Oliveira Costa, Roseane Fett

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Abstract

This study investigated the anti-inflammatory effect of hydrophilic and lipophilic extracts from juçara fruits (Euterpe edulis Martius) through measurement of nitric oxide (NOx) and cytokines (IL-12p70, TNF-α, INF-γ, MCP-1, IL-6, and IL-10). J774 macrophages were stimulated with lipopolysaccharides (1 µg/mL) and treated with various concentrations (1-100 µg/mL) of juçara fruits extracts from crude extracts, and hexane, dichloromethane, ethyl acetate, and butanol fractions. Potential relationships between the phenolic composition of the extracts determined by LC-ESI-MS/MS and their anti-inflammatory capacity were also evaluated. Hexane and dichloromethane fractions inhibited NOx and IL-12p70 while increased IL-10. Hexane fractions also decreased IL-6 and IFN-γ production. Hexane and dichloromethane fractions showed a higher number of phenolic compounds (32 and 34, respectively) than the other extracts tested and were also the only ones that presented benzoic acid and pinocembrin. These results suggest juçara fruits compounds as potential anti-inflammatory agents, especially those of a more apolar nature.

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Juçara 果实（Euterpe edulis Martius）提取物对 LPS 激活的 J774 巨噬细胞的保护作用

本研究通过测量一氧化氮（NOx）和细胞因子（IL-12p70、TNF-α、INF-γ、MCP-1、IL-6 和 IL-10），研究了亲水性和亲油性果实（Euterpe edulis Martius）提取物的抗炎作用。用脂多糖（1 µg/mL）刺激 J774 巨噬细胞，并用不同浓度（1-100 µg/mL）的茹切拉果实提取物（包括粗提取物、己烷、二氯甲烷、乙酸乙酯和丁醇馏分）进行处理。此外，还评估了通过 LC-ESI-MS/MS 测定的提取物酚类成分与其抗炎能力之间的潜在关系。正己烷和二氯甲烷馏分抑制了 NOx 和 IL-12p70，同时增加了 IL-10。正己烷馏分还能减少 IL-6 和 IFN-γ 的产生。正己烷和二氯甲烷萃取物显示的酚类化合物数量（分别为 32 和 34）高于其他测试萃取物，也是唯一显示苯甲酸和松果菊酯的萃取物。这些结果表明，Juçara 果实中的化合物具有潜在的抗炎作用，尤其是那些极性较强的化合物。

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来源期刊

Plant Foods for Human Nutrition 工程技术-食品科技

CiteScore

6.80

自引率

7.50%

发文量

审稿时长

12-24 weeks

期刊介绍： Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods