Differential expression of various isoforms of superoxide dismutase in the cells of the human exocrine pancreas.

IF 1.5 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL Bratislava Medical Journal-Bratislavske Lekarske Listy Pub Date : 2024-01-01 DOI:10.4149/BLL_2024_84
Marko Vrzgula, Jozef Mihalik, Juraj Teslik, Ingrid Hodorova
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Abstract

Objectives: Superoxide dismutase (SOD) is an enzyme that plays a crucial role in protecting cells from oxidative damage. Our study aims to address the lack of papers simultaneously analyzing the immunoreactivity of all three distinct isoforms of SOD in human exocrine pancreas cells.

Background: Superoxide dismutases (SODs) facilitate the conversion of superoxide radicals into less harmful substances. By neutralizing superoxide radicals, SODs help prevent the formation of highly reactive and destructive species that can adversely affect manifold cellular components.

Methods: The study analyzed immunoreactivity of SODs in samples of six healthy adult human pancreases, while using the indirect immunohistochemical method under a light microscope.

Results: SOD1 was predominantly found in centroacinar cells and epithelial cells of the duct system while SOD2 was mainly detected in the epithelial cells of interlobular ducts. Both enzymes were prominently present in the basal region of acinar cells near the cell nucleus. The expression of SOD3 was observed to be rare.

Conclusion: Understanding the intracellular metabolism of SODs in healthy exocrine pancreas cells serves as a basis for determining the precise role of oxidative damage and SOD signaling in the pathogenesis of various pancreatic diseases, including chronic pancreatitis and pancreatic cancer (Fig. 6, Ref. 24). Text in PDF www.elis.sk Keywords: antioxidants, histology, immunohistochemistry, pancreas, superoxide dismutase.

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人类外分泌胰腺细胞中超氧化物歧化酶各种异构体的差异表达。
目的:超氧化物歧化酶(SOD)是一种在保护细胞免受氧化损伤方面发挥关键作用的酶。我们的研究旨在解决缺乏论文同时分析人胰腺外分泌细胞中三种不同同工酶的免疫活性的问题:背景:超氧化物歧化酶(SOD)有助于将超氧自由基转化为危害较小的物质。背景:超氧化物歧化酶(SOD)可促进超氧自由基转化为危害较小的物质,通过中和超氧自由基,SOD 可帮助防止形成高活性和破坏性物种,从而对多种细胞成分产生不利影响:研究在光镜下使用间接免疫组化方法分析了 6 个健康成人胰腺样本中 SOD 的免疫活性:结果:SOD1 主要存在于中心acinar 细胞和导管系统的上皮细胞中,而 SOD2 则主要在小叶间导管的上皮细胞中检测到。这两种酶都主要存在于靠近细胞核的渐冻症细胞基底区域。SOD3 的表达很少:结论:了解健康胰腺外分泌细胞中SOD的细胞内代谢,有助于确定氧化损伤和SOD信号在各种胰腺疾病(包括慢性胰腺炎和胰腺癌)发病机制中的确切作用(图6,参考文献24)。Text in PDF www.elis.sk Keywords: antioxidants, histology, immunohistochemistry, pancreas, superoxide dismutase.
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.60
自引率
0.00%
发文量
185
审稿时长
3-8 weeks
期刊介绍: The international biomedical journal - Bratislava Medical Journal – Bratislavske lekarske listy (Bratisl Lek Listy/Bratisl Med J) publishes peer-reviewed articles on all aspects of biomedical sciences, including experimental investigations with clear clinical relevance, original clinical studies and review articles.
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