Exploring the potential of surface-active copolymers on P123 micelles for drug solubility enhancement and in vitro cytotoxicity assay

IF 2.2 4区化学 Q3 CHEMISTRY, PHYSICAL Colloid and Polymer Science Pub Date : 2024-07-09 DOI:10.1007/s00396-024-05293-4

Nitumani Tripathi, Payal Vaswani, Dhiraj Bhatia, Ketan Kuperkar, Pratap Bahadur

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Abstract

This study presents the self-assembly of polyethylene oxide (PEO)-block-polypropylene oxide (PPO)-block-polyethylene oxide (PEO)-based block copolymer (BCP) commercially known as Pluronic^® P123 in water and in the presence of different copolymeric surfactants (L61, L62, L64, and F68) with varying degree of hydrophilicity, i.e., %EO content. The clouding behavior demonstrates the varied phase transition ranging from solution, blue point (BP), and cloud point (CP). The characteristics of the micelles in the single and mix system are characterized utilizing dynamic light scattering (DLS) and small-angle neutron scattering (SANS) techniques at different temperatures. These scattering approaches provide an insightful information on the micelle size and shape. The size variations in self-assembled micelles designated the micelles to undergo growth/transition in shape from spherical to elongated ellipsoidal as function of temperature. Additionally, the application of these nanoscale micellar aggregates as carriers for delivering the anticancer drug Quercetin (QCT) was undertaken by a thorough quantitative and qualitative analysis to gain valuable insights into the effectiveness of the mix-micellar system as an ideal platform for drug delivery. A comprehensive investigation of drug release kinetics is presented using various kinetic models. The MTT (cytotoxicity assay) assay is used to gauge the effectiveness of the QCT-loaded copolymeric micelles.

Graphical abstract

Micellar transition of 5% w/v P123 in water and in the presence of copolymeric surfactants as additives at 0.2% weight fraction (fix) at different temperatures

Abstract Image

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探索 P123 胶束表面活性共聚物在提高药物溶解度和体外细胞毒性测定方面的潜力

本研究介绍了聚环氧乙烷（PEO）-嵌段-聚环氧丙烷（PPO）-嵌段-聚环氧乙烷（PEO）-基嵌段共聚物（BCP）在水中的自组装情况，以及在不同的共聚表面活性剂（L61、L62、L64 和 F68）存在下的自组装情况。混浊行为显示了从溶液、蓝点（BP）到浊点（CP）的不同相变。在不同温度下，利用动态光散射（DLS）和小角中子散射（SANS）技术对单一和混合体系中胶束的特性进行了表征。这些散射方法提供了有关胶束大小和形状的深刻信息。自组装胶束的尺寸变化表明，随着温度的变化，胶束的形状会发生从球形到细长椭圆形的增长/转变。此外，还通过全面的定量和定性分析，对这些纳米级胶束聚集体作为载体输送抗癌药物槲皮素（QCT）的应用进行了深入研究，从而对混合胶束系统作为理想的药物输送平台的有效性获得了宝贵的见解。使用各种动力学模型对药物释放动力学进行了全面研究。图解摘要5% w/v P123 在水中的胶束转变，以及共聚表面活性剂作为添加剂在不同温度下 0.2% 重量分数（固定值）的胶束转变。

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来源期刊

Colloid and Polymer Science 化学-高分子科学

CiteScore

4.60

自引率

4.20%

发文量

111

审稿时长

2.2 months

期刊介绍： Colloid and Polymer Science - a leading international journal of longstanding tradition - is devoted to colloid and polymer science and its interdisciplinary interactions. As such, it responds to a demand which has lost none of its actuality as revealed in the trends of contemporary materials science.