Externally supplied ascorbic acid moderates detrimental effects of UV-C exposure in cyanobacteria.

Abstract

The defensive role performed by exogenously supplied ascorbic acid in the cyanobacterium Nostoc muscorum Meg1 against damages produced by UV-C radiation exposure was assessed in this study. Exposure to UV-C (24 mJ/cm²) significantly enhanced reactive oxygen species (ROS) (50%) along with peroxidation of lipids (21%) and protein oxidation (22%) in the organism. But, addition of 0.5 mM ascorbic acid prior to UV-C exposure showed reduction in ROS production (1.7%) and damages to lipids and proteins (1.5 and 2%, respectively). Light and transmission electron microscopic studies revealed that ascorbic acid not only protected filament breakage but also restricted severe ultrastructural changes and cellular damages in the organism. Although the growth of the organism was repressed up to 9% under UV-C treatment within 15 days, a pre-treatment with ascorbic acid led to growth enhancement by 42% in the same period. Various growth parameters such as photo-absorbing pigments (phycoerythrin, phycocyanin, allophycocyanin, chlorophyll a, and carotenoids), water splitting complex (WSC), D1 protein, RuBisCO, glutamine synthetase and nitrogenase activities in the UV-C treated organism were seen to be relatively intact in the presence of ascorbic acid. Thus, a detailed analysis undertaken in the present study was able to demonstrate that ascorbic acid not only act as first responder against harmful UV-C radiation by down-regulating ROS production, it also accelerated the growth performance in the organism in the post UV-C incubation period as an immediate response to an adverse experience presented in the form of UV-C radiation exposure.