{"title":"Production of Preparation Rich in Zn-Pheophytin from the Leaves of Wheat Triticum aestivum L.","authors":"M. S. Khristin, T. N. Smolova","doi":"10.1134/s1021443724606116","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The aim of this work was to produce a preparation enriched in a zinc derivative of pheophytin from the excised leaves of winter wheat <i>Triticum aestivum</i> L<i>.</i> First, pheophytinization of chlorophyll was conducted, then phenolic compounds and Mg<sup>2+</sup> were washed out from the leaves at рН 3.5–4.0 with the addition of EDTA-Na followed by incubation with ZnCl<sub>2</sub>; the leaves were dried and stored in the dark. Zinc derivatives were extracted by a double treatment with ethanol. In order to improve the yield, metallization was repeated. By the results of spectrofluorimetric analysis and thin-layer chromatography, the obtained preparation corresponded to Zn-pheophytin <i>а</i> and <i>b</i>. Prospects of application of Zn-pheophytin preparation with antioxidant and antiviral activities are discussed.</p>","PeriodicalId":21477,"journal":{"name":"Russian Journal of Plant Physiology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Plant Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1021443724606116","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
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Abstract
The aim of this work was to produce a preparation enriched in a zinc derivative of pheophytin from the excised leaves of winter wheat Triticum aestivum L. First, pheophytinization of chlorophyll was conducted, then phenolic compounds and Mg2+ were washed out from the leaves at рН 3.5–4.0 with the addition of EDTA-Na followed by incubation with ZnCl2; the leaves were dried and stored in the dark. Zinc derivatives were extracted by a double treatment with ethanol. In order to improve the yield, metallization was repeated. By the results of spectrofluorimetric analysis and thin-layer chromatography, the obtained preparation corresponded to Zn-pheophytin а and b. Prospects of application of Zn-pheophytin preparation with antioxidant and antiviral activities are discussed.
期刊介绍：
Russian Journal of Plant Physiology is a leading journal in phytophysiology. It embraces the full spectrum of plant physiology and brings together the related aspects of biophysics, biochemistry, cytology, anatomy, genetics, etc. The journal publishes experimental and theoretical articles, reviews, short communications, and descriptions of new methods. Some issues cover special problems of plant physiology, thus presenting collections of articles and providing information in rapidly growing fields. The editorial board is highly interested in publishing research from all countries and accepts manuscripts in English.
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