Simultaneous estimation of rutin and donepezil through RP-HPLC: implication in pharmaceutical and biological samples.

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS Bioanalysis Pub Date : 2024-06-02 Epub Date: 2024-07-16 DOI:10.1080/17576180.2024.2344395

Rafquat Rana, Keerti Mishra, Shourya Tripathi, Animesh Kumar Gupta, Amrendra Kumar Tiwari, Pavan Kumar Yadav, Abhiram Kumar, Jvus Chakradhar, Sanjay Singh, Sonia Verma, Pooja Yadav, Manish K Chourasia

引用次数: 0

Abstract

Aim: A HPLC method was developed and validated for the novel combination of rutin (RN) and donepezil (DNP). Materials & methods: RN and DNP were simultaneously eluted through a C18 column (Ø 150 × 4.6 mm) with a 60:40 v/v ratio of 0.1% formic acid aqueous solution to methanol at 0.5 ml/min. Results: The purposed method was found linear, selective, reproducible, accurate and precise with percent RSD less than 2. The limit of quantification for RN and DNP was found 3.66 and 3.25 μg/ml, respectively. Conclusion: Validated as per the ICH guidelines, the developed method efficiently quantified RN and DNP co-loaded in DQAsomes (121 nm) estimating matrix effect, release profile, entrapment efficiency, loading efficiency and in vivo plasma kinetics.

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通过 RP-HPLC 同时估算芦丁和多奈哌齐：对药物和生物样品的影响

目的：建立并验证了芦丁（RN）和多奈哌齐（DNP）新型复方制剂的高效液相色谱法。材料与方法：RN和DNP同时经C18色谱柱（直径150×4.6 mm）洗脱，0.1%甲酸水溶液与甲醇的体积比为60:40，流速为0.5 ml/min。结果表明RN 和 DNP 的定量限分别为 3.66 和 3.25 μg/ml。结论根据 ICH 指南验证，所开发的方法能有效定量 DQAsomes（121 nm）中共载的 RN 和 DNP，并能估计基质效应、释放曲线、夹带效率、负载效率和体内血浆动力学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.